生长相关蛋白-43在间质性膀胱炎患者膀胱黏膜表达的研究

The expression of growth associated protein 43 in bladder tissue of patients with interstitial cystitis

目的探讨生长相关蛋白43(GAP-43)在间质性膀胱炎/膀胱疼痛综合征(IC/BPS)患者膀胱黏膜组织中的表达情况,以及与患者症状严重程度之间的相关性。方法通过免疫组织化学法检测GAP-43在31例IC/BPS患者膀胱组织中的表达情况,并通过入院时对全部病例进行症状评分(ICSI),问题评分(ICPI)和盆腔疼痛、尿频/尿急症状评分(PUF),评估GAP-43的表达与IC/BPS患者症状严重性之间的相关性。结果 31例IC/BPS患者中,ICSI 13~19分,平均(16±3)分;ICPI7~16分,平均(12±1)分。PUF 16~25分,平均(20±2)分。症状总分37~57分,平均(46±4)分。IC/BPS患者膀胱组织中GAP-43表达水平免疫组化评分为0~6分,平均(5.2±0.3)分。GAP-43在IC/BPS患者膀胱组织中的表达程度与患者的症状严重程度(ICSI+ICPI、PUF、ICSI+ICPI+PUF)之间成正相关(相关系数=0.469、0.611、0.426),具有统计学意义(P=0.046、0.039、0.008)。结论 GAP-43在IC/BPS患者膀胱组织中的表达与患者的症状严重程度成正相关,提示GAP-43在IC/BPS的发病机制中可能起着一定的作用。

Objective To explore the growth associated protein （GAP-43） expression in patients with interstitial cystitis/bladder painful syndrome （IC/BPS） and the correlation between symptoms. Methods Through the immune histochemical method to detect GAP-43 expression in bladder tissue of 31 patients with IC/BPS, ICSI, ICPI and PUF score before treatment, and the ralationship between GAP- 43 expression and patient＇s symptoms were assessed. Results Of 31 IC/BPS patients, ICSI scores were ranged from 13 to 19 （mean 16±3）, ICPI scores were ranged from 7 to 16 （mean 12±1）, PUF scores were ranged from 16 to 25 （mean 20±2）, symptom scores were ranged from 37 to 57 （46±4）. The expression of GAP-43 immune histochemical scores were ranged from 1 to 6 （mean 5.2 ± 0.3） points. There was a positive correlation between GAP-43 expression levels in the bladder tissue and symptom severity of IC/BPS patients, correlation coefficients were 0.469, 0.611, 0.426 respectively, P value were 0.046, 0.039, 0.008 respectively. Conclusion GAP-43 expression in bladder tissue of IC/BPS patients are positively related to the patient＇s symptom severity, suggesting that GAP-43 expression in bladder tissue may be involved in the pathogenesis of IC/BPS.