慢性前列腺炎TGF-β1、CTGF的表达水平及其与症状指数的关系

Expression of TGF-β1 and CTGF in chronic prostatitis and its correlation with chronic prostatitis symptom index

目的探讨慢性前列腺炎(CP)转化生长因子-β1(TGF-β1)、结缔组织生长因子(CTGF)的表达水平,分析其与临床症状的关系。方法根据美国国立卫生研究院慢性前列腺症状指数(NIH-CPSI),将86例CP患者分为轻度组(38例)、中度组(31例)、重度组(17例),另选择健康体检者作为对照组(30例)。采用酶联免疫吸附法(ELISA)检测前列腺液(EPS)中白细胞(WBC)计数、TGF-β1和CTGF的表达水平,分析NIH-CPSI、EPS-WBC、TGF-β1和CTGF之间的相关性。结果与对照组比较,CP组EPS-WBC计数中++^++++比例明显增高(P<0.05);不同NIH-CPSI评分的CP患者EPS-WBC计数比较,差异无统计学意义(P>0.05);Ⅱ型CP患者EPSWBC计数++^++++比例显著高于Ⅲ型(P<0.05)。与对照组比较,CP组TGF-β1、CTGF表达水平均有不同程度升高,随着症状严重程度的增加,TGF-β1和CTGF表达水平也显著升高(P<0.05);TGF-β1与CTGF的表达水平呈显著正相关,TGF-β1、CTGF的表达水平与NIH-CPSI呈显著正相关(P<0.05);GF-β1、CTGF与EPS-WBC无显著相关性(P>0.05)。结论 CP患者TGF-β1、CTGF表达水平明显升高,且与NIH-CPSI明显相关,联合检测有助于评估CP患者的病情严重程度。

Objective To explore the expression of TGF-β1 and CTGF in chronic prostatitis （CP） and its correlation with chronic prostatitis symptom index （CPSI）. Methods WBC, TGF-β1 and CTGF from expressed prostatic secretion （EPS） were detected by ELISA in mild group （n=38）, moderate group （n=31）, severe groups （n= 17）, and control group （n=30） according to the NIH-CPSI. The correlation among NIH-CPSI, EPS-WBC, TGF-β1 and CTGF were analyzed. Result Compared with control group, the proportion of EPS-WBC （＋＋- ＋＋＋＋） count in CP group was significantly increased （P〈0.05）. There was no significant difference on the EPS-WBC count among different NIH-CPSI score of CP patients （P〉0.05）. The proportion of EPS-WBC （＋＋- ＋＋＋＋） count in type II of CP group was significantly higher than that in type II （P〈0.05）. Compared with control group, the levels of TGF-β1 and CTGF also increased CP group.With the increase of symptom severity, levels of TGF-β1 and CTGF was also significantly increased （P〈0.05）. There was a positive correlation between levels of TGF-β1 and CTGF, levels of TGF-β1 and CTGF was positively correlated with NIH-CPSI （P〈0.05）.The levels of TGF-β1 and CTGF had no significant correlation with EPS-WBC count （P〉0.05）. Conclusions The levels of TGF-β1 and CTGF in CP patients are highly expressed, and closely correlated with NIH-CPSI. Combination detection of TGF-β1and CTGF may contribute to evaluate the severity of CE