猴头菌丝固体培养物中3个核苷类成分含量测定及HPLC指纹图谱研究

Content of 3 nucleosides and HPLC fingerprint of solid culture of Hericium mycelium

目的:对猴头菌丝固体培养物中尿苷、鸟苷、腺苷3个核苷类成分进行含量测定,同时建立HPLC指纹图谱。方法:采用SunFire^(TM) C_(18)色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇-水,梯度洗脱,流速1.0 m L·min^(-1),检测波长260 nm,柱温30℃。采用中药色谱指纹图谱相似度评价系统软件(2004A)进行指纹图谱分析。结果:10批猴头菌丝固体培养物样品中尿苷、鸟苷、腺苷3个核苷类成分线性范围分别为2.4~38.9、1.9~29.6、1.7~26.9μg·m L^(-1),平均加样回收率分别为97.8%、98.4%、99.0%,含量分别在127.17~481.88,68.36~282.36,91.10~280.47μg·g^(-1)范围内。建立了10个批次猴头菌丝固体培养物的共有图谱,提取了21个色谱峰作为指纹图谱共有峰,指纹图谱相似度在0.93以上。结论:建立的猴头菌丝固体培养物含量测定及指纹图谱方法简便可靠,重复性好,可为猴头菌丝固体培养物质量控制和评价提供依据。

Objective：To determine 3 nucleosides in solid culture of Hericium mycelium including uridine,guanosine and adenosine,and to establish the HPLC fingerprint.Methods：The separation was developed on a Sun Fire^TM C18 column（4.6 mm×250 mm,5 μm）by gradient elution with methanol and water at a flow rate of 1.0 mL·min^-1.The detection wavelength was set at 260 nm and the column temperature was set at 30 ℃.The data calculation was performed with Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine（Version 2004 A）.Results：Good linearities of uridine,guanosine and adenosine were observed in the ranges of 2.4-38.9,1.9-29.6 and 1.7-26.9 μg·mL^-1,respectively.The average recoveries were 97.8%,98.4% and 99.0%,respectively.The content ranges of uridine,guanosine and adenosine were 127.17-481.88,68.36-282.36 and 91.10-280.47 μg·g^-1,respectively.The common pattern was established based on fingerprints of 10 batches of solid culture of Hericium mycelium and 21 chromatographic peaks were designated as the common peaks.The similarities of samples were above 0.93.Conclusion：The established methods are simple,reliable and repeatable,which can provide the basis for the evaluation and control of the quality of solid culture of Hericium mycelium.