小麦淀粉加工废液制备葡萄糖浆的糖化工艺优化

Optimization of the saccharification process of glucose from waste water produced during wheat starch processing

以降黏和液化后的小麦加工副产物为原料,将原料中的小麦淀粉制备为葡萄糖浆,并优化其糖化工艺以提高葡萄糖的回收率。探究葡萄糖淀粉酶添加量、普鲁兰酶添加量、糖化时间、糖化温度和糖化p H各单因素对糖化液还原糖含量(DE值)的影响。通过响应面实验得出最优工艺参数为葡萄糖淀粉酶添加量300 U/g、普鲁兰酶添加量0.17 U/g、糖化温度62℃、糖化p H3.9,糖化时间36 h,得到的DE值为69.45%。进一步通过高效液相-蒸发光散射检测法,测出淀粉转化率为78.70%,葡萄糖总回收率为23.82%。说明从小麦加工废液中制取葡萄糖浆是切实可行的,对实际生产有着重要的指导意义。

In this paper, by using the byproducts of wheat processing after decreasing viscosity and liquefaction, the wheat starch from byproducts was prepared as a glucose syrup. The addition of glucoamylase, the amount of pullulanase, the saccharification temperature and the saccharification pH＇ s effect on the DE value were studied. The optimum technique parameters were obtained by using responses surface tests： the amount of glucose amylase was 300 U/g, the amount of pullulanase was 0.17 U/g,the saccharification temperature was 62 ℃ ,the saccharification pH was 3.9,the saccharification time was 36 h,and the DE value was 69.45% , and the effluent conversion rate was 78.70% and the total recovery of glucose was ？,3.82% by high performance liquid-evaporative light scattering test.This approves that it was practicable to produce glucose syrup from the byproduct of wheat processing, and it had important guiding significance for practical production.