响应面法优化蚕豆多酚超声辅助提取工艺

Optimization of ultrasound-assisted extraction technology of polyphenols from broad bean by response surface methodology

为了研究超声波辅助提取蚕豆多酚的最佳工艺条件,在单因素实验的基础上,选取乙醇体积分数、料液比、提取时间、p H为自变量,多酚提取量为响应值,根据Box-Benhnken实验设计原理,采用四因素三水平的分析法对蚕豆多酚提取量进行优化。结果表明,超声波辅助提取蚕豆多酚的最佳工艺条件为:乙醇体积分数45%、料液比1∶25(g/m L)、提取时间20 min、p H5,此条件下蚕豆多酚提取量最高为(2.03±0.16)mg/g d.w.,与预测值(2.06±0.23)mg/g d.w.相近,说明该优化方法合理可行。

The aim of this work was to optimize the ultrasound-assisted extraction technology of polyphenols from broad bean. On the basis of single-factor test, the response surface experiments with four factors and three levels was further utilized to investigate the effects of processing parameters,including ethanol content, solvent/material ratio,extraction time and pH on the yield of polyphenols. Results showed that the optimal extraction parameters were as follows： ethanol content 45%, solvent/ material ratio 1：25 （ g/mL）, extraction time 20 min, and pH5.Under the optimized condition, the yield of polyphenols was up to （2.03 ± 0.16） mg/g d.w., which was consistent with the predicted value of（2.06±0.23 ） mg/g d.w.. Therefore, the optimized extraction method was considered to be reliable.