葵花籽粕多肽酶解液的精制

Refining of sunflower seed meal peptide hydrolyzate

为了利用较为优质的植物性蛋白葵花籽粕蛋白质,在微波酶解条件下,运用单因素确定了葵花籽粕抗氧化多肽液的最适脱盐条件为:酶解液p H4.5、阴阳离子树脂比例3∶2、过柱速度8倍柱/h。在此条件下,脱盐率为84.54%、·OH和O_2^-·的清除率分别为43.62%和57.08%。最适超滤条件为:超滤压力0.16 MPa、液体流速2.5 m L/s、超滤时间60 min。在此条件下,渗透通量为25.98 L/m^2·h、膜污染度为0.021、·OH和O_2^-·的清除率分别为73.02%和86.58%。通过对葵花籽粕蛋白酶解液进行了深入的研究,为葵花籽粕多肽产品的开发和工业化批量生产功能性生物活性肽产品提供了理论依据。

In order to make use of the higher quality plant protein sunflower seed meal protein, under the condition of microwave enzymolysis, using single factor to determine the optimal desalination conditions for antioxidant peptide fluid sunflower seed meal as follows ： hydrolysis pH4.5, anion and cation resin ratio 3 ： 2, through column speed 8 times eolumn/h. Under this condition, the rate of desalination is 84.54% , the clearance rates of· OH and O2-·were 43.62% and 57.08% respectively.Ultrafiltration pressure 0.16 MPa, liquid flow rate 2.5 mL/s, ultrafiltration time 60 rain. Under this condition, permeate flux was 25.98 L/m2· h, membrane fouling was 0.021 , the clearance rates of · OH and O2 -· were 73.02% and 86.58% ,respectively.Through experiments, the protease solution of sunflower seed meal was studied in depth, it provides a theoretical basis for the development of the sunflower seed meal peptide product and the industrialized batch production of the functional bioactive peptide product.