超临界CO_2萃取阿萨伊油的工艺优化及GC-MS分析

Optimization of supercritical CO_2 extraction process of oil from Euterpe oleraceae Mart.and analysis by GC-MS

本文以阿萨伊冻干粉为原料,以油脂得率为指标,通过考察萃取压力、萃取温度、萃取时间对指标的影响进行单因素实验和正交实验,确定超临界CO_2流体萃取技术提取阿萨伊油的最佳条件,并用气相色谱-质谱联用技术对其成分进行分析。研究表明,阿萨伊油的最佳提取工艺为:萃取压力25 MPa,萃取温度40℃,萃取时间90 min。在此条件下,阿萨伊油的得率为54.871%,从阿萨伊油中共分离鉴定出34种化合物,其中含量较高的为油酸、棕榈酸、亚油酸、棕榈油酸、硬脂酸和亚麻酸,相对百分含量分别为44.607%、19.028%、14.328%、6.702%、6.123%、2.067%。该研究为阿萨伊的综合开发利用及质量评价提供了理论依据。

In this paper, Acai freeze- dried powder was used as the raw material, oil yield as the index for investigation, the effects of extraction pressure, extraction temperature, extraction time were investigated by single- factor experiments, and Supercritieal CO2 fluid extraction process of Acai oil was optimized by orthogonal design.The composition was analyzed by gas chromatography-mass spectrometry （GC-MS）. The optimum extraction conditions were as follows： extraction pressure was 25 MPa, extraction temperature was 40 ℃ , extraction time was 90 min, and the extraction rate of Acai oil was 54.871% .Under these conditions ,34 compounds were isolated and identified, and the higher content was palmitic acid, palmitic acid, stearic acid, oleic acid, linoleic acid and linolenic acid. The relative content were 44.607%, 19,028%, 14.328%,6.702%,6.123% and 2.067% respectively. This study provides the basis for the comprehensive development, utilization and quality evaluation of Acai.