miRNA-34b/c-5p对乳腺癌中神经激肽1受体-截短型表达的影响

The effect of miRNA-34b/c-5p on neurokinin-1 receptor-truncated expression in breast cancer

目的:探究乳腺癌中miRNA-34b/c-5p对神经激肽1受体-截短型(neurokinin1 receptor-truncated,NK1R-Tr)表达的影响及miRNA-34b/c-5p和NK1R-Tr对乳腺癌细胞迁移侵袭能力的影响。方法:收集2013年2月至5月天津医科大学肿瘤医院50例乳腺癌患者组织标本,采用实时荧光定量PCR(RT-PCR)检测miRNA-34b/c-5p及NK1R-Tr的表达,采用蛋白免疫印迹实验检测乳腺癌MDA-MB-231和MCF-7细胞中miRNA-34b/c-5p对NK1R-Tr表达的影响,采用划痕及Transwell实验检测miRNA-34b/c-5p及NK1R-Tr对MDA-MB-231细胞迁移及侵袭能力的影响。结果:在乳腺癌组织及MDA-MB-231和MCF-7细胞中,miRNA-34b-5p和miRNA-34c-5p与NK1R-Tr表达呈负相关。发生淋巴结转移的乳腺癌患者组织中NK1R-Tr的相对表达量为5.75,未发生淋巴结转移者相对表达量为4.29,两者比较差异具有统计学意义(P=0.026)。过表达miRNA-34b/c-5p及敲低NK1R-Tr可以显著抑制MDA-MB-231细胞的迁移侵袭能力(均P<0.001)。结论:乳腺癌中miRNA-34b/c-5p与NK1R-Tr表达呈负相关,且miRNA-34b/c-5p和NK1R-Tr可能成为抑制乳腺癌转移的潜在治疗靶点。

Objective： To investigate the effect of miRNA-34 b/c-5 p on the expression of neurokinin 1 receptor-truncated（NK1R-Tr） in breast cancer and the effect of miRNA-34 b/c-5 p and NK1R-Tr on the migration and invasion abilities of breast cancer cells. Methods：Real-time fluorescence quantitative PCR（RT-PCR） was used to detect the expression of miRNA-34 b/c-5 p and NK1R-Tr in 50 breast cancer specimens that were collected at Tianjin Medical University Cancer Institute Hospital from February to May 2013. Western blot analysis was performed to detect the expression of miRNA-34 b/c-5 p and NK1R-Tr in breast cancer cell lines MDA-MB-231 and MCF-7.Scratch and Transwell assays were carried out to explore the effects of miRNA-34 b/c-5 p and NK1R-Tr on the migration and invasion abilities of MDA-MB-231 cells. Results： The expression of miRNA-34 b/c-5 p and NK1R-Tr in breast cancer tissues and cells were significantly negatively correlated. The relative expression of NK1R-Tr in breast cancer patients with lymph node metastasis was 5.75, and the relative expression of NK1R-Tr in patients with non-lymph node metastasis was 4.29. The relative expression was significantly different between the two groups（P=0.026）. Overexpression of miRNA-34 b/c-5 p and knockdown of NK1R-Tr could significantly inhibit the migration and invasion abilities of MDA-MB-231 cells（all P0.001）. Conclusions： The expression of miRNA-34 b/c-5 p and NK1R-Tr was significantly negatively correlated. Mi RNA-34 b/c-5 p and NK1R-Tr might be potential therapeutic targets for inhibiting the invasion of breast cancer cells.