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胃腺癌组织中UCP2的表达及其对癌细胞生物学功能的影响 被引量:5

Expression of UCP2 in Gastric Adenocarcinoma Tissues and Its Effect on Biological Function of Gastric Adenocarcinoma Cells
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摘要 目的分析UCP2(uncoupling protein-2)在胃腺癌组织中的表达,探讨UCP2表达与胃腺癌临床病理特征及患者预后的关系,研究UCP2对胃癌细胞生物学功能的影响。方法免疫组织化学法检测142例胃腺癌及25例癌旁正常胃黏膜组织中UCP2的表达情况,Pearsonχ2检验分析其与临床病理特征的关系,Kaplan-Meier法及Cox风险回归模型分析UCP2的表达与胃腺癌患者预后生存的关系。将UCP2 si RNA转染SGC7901胃腺癌细胞系后,Western blot法检测UCP2蛋白的表达,Ed U实验检测细胞增殖,划痕实验检测细胞迁移能力。结果在胃腺癌组织中UCP2蛋白的阳性表达率明显高于癌旁正常胃黏膜组织(P=0.006),UCP2蛋白的表达与肿瘤浸润深度(P=0.024)、TNM分期(P=0.002)、淋巴结转移(P=0.001)显著相关;UCP2阳性患者5年生存率明显低于阴性患者(P=0.001)。在胃腺癌SGC7901细胞系中,UCP2高表达,沉默UCP2的表达能显著抑制肿瘤细胞的增殖,同时降低其迁移能力。结论 UCP2阳性表达是胃腺癌患者预后不良的重要因素,UCP2能显著促进胃腺癌细胞的增殖和迁移。 Objective To analyze the expression of uncoupling protein-2(UCP2) in human gastric adenocarcinoma tissues and investigate its correlation with the clinical pathological features and prognosis of gastric cancer patients, and to explore its effect on the proliferation and migration of gastric adenocarcinoma cells. Methods Totally 142 cases of gastric cancer and randomized 25 cases of normal gastric mucosa tissues in Tianjin Medical University Cancer Institute and Hospital were included. We used immunohistochemical staining to detect the expression of UCP2 and analyze its relationship with clinical pathological features. Kaplan-Meier method and Cox regression analysis were used to explore the correlation between the expression of UCP2 and the survival of gastric cancer patients. Western blot was used to detect UCP2 protein level after UCP2 si RNA transfection into SGC7901 gastric cancer cell lines. Ed U assays and scratch assay were used to detect the proliferation and migration abilities. Results The expression of UCP2 in gastric adenocarcinoma tissues was significantly higher than that in adjacent normal tissues(65.5% vs. 28%, P=0.006). The expression of UCP2 in gastric adenocarcinoma tissues was significantly correlated with tumor infiltration(P=0.024), TNM stage(P=0.002) and lymph node metastasis(P=0.001). The 5-year overall survival of patients with positive UCP2 expression was significantly lower than those without UCP2 expression(P=0.001). Silencing UCP2 expression could significantly inhibit the proliferation and migration abilities of SGC7901 cell lines. Conclusion Positive UCP2 expression is a prognostic factor of gastric adenocarcinoma patients. UCP2 could stimulate the proliferation and migration of gastric cancer cells.
作者 王艺 张海洋 应国光 巴一 WANG Yi;ZHANG Haiyang;YING Guoguang;BA Yi(Department of Gastrointestinal Oneology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, Tianjin 's Clinical Research Center for Cancer, Tianjin 300060, Chin)
出处 《肿瘤防治研究》 CAS CSCD 2018年第4期215-220,共6页 Cancer Research on Prevention and Treatment
基金 国家自然科学基金(81602158 81602156)
关键词 胃腺癌 UCP2 预后 增殖 侵袭 Gastric adenocarcinoma UCP2 Prognosis Proliferation Migration
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