MicroRNA相关基因Dicer rs1057035多态性与脑胶质瘤的相关性研究

Association of the microRNA-related gene Dicer rs1057035 polymorphism and glioma

目的探讨陕西地区汉族人群中,Dicer基因rs1057035多态性与脑胶质瘤的相关性。方法 200例陕西地区脑胶质瘤患者作为病例组,随机选择同期在我科住院治疗的非肿瘤疾病患者184例作为对照组,收集所有入住患者相关临床资料,并采用聚合酶链反应-限制性内切酶分析(PCR-RFLP)法检测Dicer基因rs1057035单核苷酸多态性。结果病例组患者Dicer基因rs1057035位点等位基因C分布频率明显低于对照组(χ~2=5.137,P=0.023)。分层分析,携带突变等位基因C可降低酗酒者,无肿瘤性疾病家族史者以及Ⅲ型、Ⅳ型胶质瘤患者发病风险(OR=0.253,95%CI 0.067～0.949,P=0.036;OR=0.561,95%CI 0.339～0.930,P=0.024;OR=0.525,95%CI 0.284～0.969,P=0.037)。结论 Dicer基因rs1057035多态性可能与脑胶质瘤遗传易感性相关。

Objective To investigate the association between the Dicer gene rs1057035 polymorphism and glioma in the Han Chinese population in Shaanxi Province, China. Methods A total of 200 patients with glioma in Shaanxi Province were enrolled as case group, and 184 patients without tumor diseases who were hospitalized and treated in our department during the same period of time were enrolled as control group. Their clinical data were collected, and polymerase chain reaction-restriction fragment length polymorphism was used to analyze the single nucleotide polymorphism of Dicer gene rs1057035. Results The case group had a significantly lower frequency of C allele of Dicer gene rs1057035 than the control group （X2 = 5. 137, P = 0.023 ）. The stratified analysis showed that Dicer rs1057035 C allele significantly reduced the risk of glioma in alcoholics （odds ratio [ OR] = 0. 253, 95% confidence interval [ CI] ： 0.067 - 0. 949, P = 0.036 ）, patients without a family history of tumor disease （ OR = 0.561, 95% CI： 0. 339 - 0.930, P = 0.024 ）, or patients with type III/IV glioma （ OR =0.525, 95% CI： 0. 284 -0. 969, P =0.037）. Conclusions The Dicer rs1057035 polymorphism may be associated with the genetic susceptibility to glioma.