摘要
以木薯未成熟叶为花青素(以矢车菊素代表)提取原料,研究其在可见光条件下的最高吸收波长和不同提取剂的提取效果,并采用单因素及正交试验法分别对提取条件进行分析,利用SAS软件进行提取工艺条件优化。结果表明木薯叶花青素最佳可见光吸收波长为533 nm,提取剂为酸性甲醇时提取效果好,最优提取工艺为:以酸性甲醇为提取溶剂,料液比为1:20,提取温度为60℃,提取时间为4 h,在此条件下,提取的木薯叶花青素含量为158.78 mg/hg。
Cassava immature leaves were subjected to analyze the conditions of the extraction for anthocyanin （represented by cyanidin） based the orthogonal experiment method and optimized extraction conditions by SAS software. Results showed that the best visible light absorption wavelength was 533nm based the acid methanol extraction agent and the optimum conditions for the extraction of anthocyanins were as following： with acidic methanol as the extraction solvent, liquid ratio 1：20, extraction temperature 60 ℃, and the extraction time 4 hours. Under the condition, an anthocyanin extraction rate up to 158.78 mg/hg could be achieved.
作者
张振文
徐缓
吴秋妃
林立铭
李开绵
ZHANG Zhenwen;XU Huan;WU Qiufei;LIN Liming;LI Kaimian(Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/National R＆D Genter for Potato Processing/ Key Laboratory of Conservation and Utilization of Cassava Genetic Resources, Ministry of Agriculture, Danzhou, Hainan 571737, China)
出处
《热带作物学报》
CSCD
北大核心
2018年第3期570-574,共5页
Chinese Journal of Tropical Crops
基金
海南省应用技术研发与示范推广专项(No.SQ2015YFSF0038)
现代农业产业技术体系(No.CARS-11)
非盈利启动项目(No.pzsfyl-201718)
关键词
木薯叶
正交实验
花青素
提取工艺
cassava leaves
orthogonal design
anthocyanin
extraction process
