SWATH定量蛋白质组学揭示光酶诱导桑叶及桑辛素N对家蚕中肠组织的影响

Proteomic analysis of midgut in silkworm reared on UV-B induced mulberry leaves and moracin N by SWATH-MS

桑是一种重要的药用植物,桑叶是家蚕的主要食物来源.前期研究表明,桑叶经光酶诱导后次生代谢产物的含量发生变化,其中桑辛素N的含量显著增加.为了研究饲喂不同桑叶对家蚕生长发育的影响,本文应用了SWATH定量蛋白质组学技术对饲喂新鲜桑叶(FM)、饲喂光酶诱导桑叶(UVM)及饲喂涂布桑辛素N(NM)的桑叶家蚕中肠组织进行蛋白质表达谱的差异分析.结果表明,与FM组相比,在UVM组中共鉴定到90个差异蛋白,而在NM组中共鉴定到182个差异蛋白.其中与能量代谢相关蛋白液泡ATP合酶上调表达17倍,与家蚕抗病性相关的蛋白如ABC转运蛋白、PDCD6IP、核糖体蛋白等均有不同程度上调表达.GO注释和KEGG pathway分析结果表明,饲喂诱导桑叶或涂布桑辛素N的桑叶会影响家蚕氨基酸代谢和丙酸代谢.因此,光酶诱导桑叶及桑辛素N有可能发展成为一种潜在的药物用于提高家蚕的免疫力.

Mulberry is a kind of important medicinal plant and its leaf is the main food sources of silkworm. Previous studies showed that the content of secondary metabolites in mulberry leaves, particularly for moracin N, was increased after UV-B induction. To uncover the effects on the growth and development in silkworm reared on different mulberry leaves, SWATH-based proteomic technique was used to analyze the protein profiles of midgut in silkworm reared on fresh mulberry leaf （FM）, UV-B induced mulberry leaf （UVM）, and fresh mulberry leaf spread with moracin N （NM）, respectively. Compared to the FM group, a total of 90 and 182 proteins were identified as differentially expressed proteins in UVM and NM groups, respectively. Of note, the abundance of vacuolar ATP synthase protein was increased by 17 times. The abundance of proteins related to silkworm disease resistance such as ATP-binding cassette, programmed cell death 6-interacting protein, and ribosomal protein were also increased. GO category and KEGG pathway analysis showed that amino acid metabolism and propanoate metabolism in silkworm were affected by UVM and NM. These results suggest that UVM and NM could be developed as a potential drug for improving the immune system in silkworm.