β-榄香烯对人食管癌细胞Eca-109增殖、凋亡和迁移的影响

Effect of β-elemene on proliferation,apoptosis and migration in human esophageal carcinoma cell Eca-109

目的检测β-榄香烯对人食管鳞癌细胞Eca-109增殖、凋亡和迁移的影响,初步探讨其对食管癌细胞的作用机制。方法采用不同浓度的β-榄香烯(终浓度分别为0μg/m L、1μg/m L、20μg/m L、100μg/m L、200μg/m L、400μg/m L、600μg/m L)作用于Eca-109细胞后,CCK-8测定细胞增殖活力。同时采用Annexin V/PI双染法(β-榄香烯作用浓度为0μg/m L、10μg/m L、100μg/m L)检测细胞的凋亡率(流式细胞仪)。此外,采用β-榄香烯(0μg/m L、100μg/m L)作用细胞后,检测其对细胞迁移的影响(榄香烯浓度为0μg/m L组作为对照组)。β-榄香烯作用于细胞均为48 h。结果β-榄香烯孵育48 h后对食管癌细胞增殖抑制在不同浓度之间比较,差异均有统计学意义(P<0.05),同时,随着浓度增加,β-榄香烯对细胞的增殖抑制进一步增强,且其抑制作用存在剂量依赖性;不同浓度β-榄香烯对细胞的凋亡率间比较,差异均有统计学意义(P<0.05);相对于对照组,β-榄香烯显著降低迁移细胞数,两者间差异亦有统计学意义(P<0.05)。结论β-榄香烯显著抑制了Eca-109细胞的增殖活力,且其抑制存在剂量依赖性,同时可诱导凋亡及抑制细胞迁移,可能通过诱导细胞凋亡及抑制迁移发挥其抗肿瘤效应。

Objective To investigate the effect of β-elemene on the proliferation, apoptosis and migration of hu-man esophageal squamous carcinoma cell Eca-109, and to explore its mechanism of anti-tumor effect. Methods Eca-109cells were incubated with different concentrations of β-elemene （final concentration to 0 μg/mL, 1 μg/mL, 20 μg/mL,100 μg/mL, 200 μg/mL, 400 μg/mL, 600 μg/mL） for 48 h, then cholecystokinin octapeptide （CCK-8） assay was used to de-tect the cell proliferation activity. For the apoptosis detection, Annexin-V/PI assay was used and the cells were detectedon the flow cytometry before incubation with different concentrations of β-elemene （final concentration to 0 μg/mL,10 μg/mL and 100 μg/mL）. While for the migration activity detection, the concentration of 0 μg/mL and 100 μg/mL ofβ-elemene were set as the control and experimental group respectively. Results After the incubation of 48 h for β-el-emene, the inhibition of cell proliferation were significant different among different concentrations of β-elemene （P〈0.05）.Meantime, with the increase of concentration, the inhibition of cell proliferation was further enhanced by β-elemene, andits inhibitory effect was dose-dependent. The cell apoptosis were significant different among different concentrations ofβ-elemene （P〈0.05）. Compared with the control group, β-elemene significantly reduced the number of migratory cells （P〈0.05）. Conclusion The proliferation of cell line of Eca-109 can be inhibited by β-elemene and the inhibited rate isdose-dependent. β-elemene may make its anti-tumor effect through inducing the cell apoptosis and migration.