去卵巢老年大鼠软骨退变与E_2、MMP-13、TNF-α的相关性

Correlation between E_2, MMP-13, TNF-α and Degeneration of Cartilage among Ovariectomized Aged Rats

目的探讨去卵巢老年大鼠雌激素下降与软骨退变的相关性。方法 24只6月龄雌性SD大鼠按数字表法随机分为对照组、去卵巢组和去卵巢+跑步组各8只。对照组常规饲养,去卵巢组行双侧卵巢切除术,去卵巢+跑步组行双侧卵巢切除术合并疲劳训练。5周后处死,取左侧膝关节石蜡包埋,行HE染色、免疫组化分析、膝关节软骨Mankin评分,右侧膝关节抽取关节液检测TNF-α含量,取血清检测E_2含量。结果 (1)膝关节切片HE染色显示:与对照组比较,去卵巢组与去卵巢+跑步组膝关节软骨均出现缺损,其中去卵巢+跑步组缺损最重;(2)与对照组比较,去卵巢组和去卵巢+跑步组mankin评分、膝关节MMP-13表达、关节液TNF-α浓度均明显提高(P<0.01),血清E_2浓度明显降低(P<0.01),且以去卵巢+跑步组变化最为显著。结论老年大鼠雌激素的下降与软骨退变具有一定的相关性。

Objective： To investigate the relationship between estrogen decline and cartilage degeneration among ovariectomized aged rats. Methods： Twenty-four female 6-month-old SD rats were randomly divided into control group, ovariectomized group and ovariectomized ＋ running group according to random number table. The control group was fed with conventional diet, the ovariectomized group was treated with bilateral ovariectomy, and the ovariectomized＋running group received bilateral ovariectomy combined with fatigue training. Rats were executed after 5 weeks, and their left knees were treated with paraffin-embedded, HE staining, immunohistochemical analysis, knee joint cartilage mankin score. The synovial fluid of right knee joint extract was extracted to detect the TNF-α, and E2 was determined through the serum. Results： （1） HE staining of knee joint section showed that there were defects in articular cartilage in ovariectomized group and ovariectomized ＋running group, compared with the control group, especially in ovariectomized running group. （2） Compared with the control group, Mankin score, expression of MMP-13 in knee joint and the concentration of TNF-α of synovial fluid in ovariectomized group and ovariectomized＋ running group were significantly increased （P〈 0.01, P〈0.05）. Serum E2 concentration was significantly decreased （P〈0.01, P〈0.05）. Conclusion： The decline of estrogen in old rats is related to the degeneration of cartilage.