菲胁迫对鲤鱼的急性毒性和抗氧化酶响应

Acute Toxicity and Response of Antioxidant Enzymes of Phenanthrene Stress on Carp

为探究多环芳烃类污染物的生物毒性作用,对鲤鱼进行菲的急性毒性试验,并根据测得的96h半致死浓度(96h-LC50)设置0.175、0.350、0.700、1.400、2.800、5.600mg/L 6个菲浓度组进行亚急性毒性试验(暴露时间为1、3、5、7、9d),暴露后测定鲤鱼鳃、肝脏、肌肉组织中的超氧化物歧化酶(SOD)和谷胱甘肽(GSH)的活力。结果表明:菲对鲤鱼的24h半致死浓度(24h-LC50)为15.926mg/L,96h-LC50为11.198mg/L,安全质量浓度为1.120mg/L;菲对鲤鱼的亚急性毒性作用显著,其鳃和肌肉中SOD表现出诱导效应,肝脏组织中表现出低浓度诱导、高浓度抑制-诱导效应;GSH响应趋势与SOD相似。试验结果可为多环芳烃污染的生物标志物研究提供依据。

To investigate the biological toxicity of polycyclic aromatic hydrocarbons（PAHs）,the carp was firstly exposed for acute toxicity test with phenanthrene.According to the measured 96 h-LC50 results of acute toxicity test,a series of phenanthrene concentration groups（0.175,0.350,0.700,1.400,2.800,5.600 mg/L）were performed for the subacute toxicity test on carp with 1,3,5,7,9 d.The activity of superoxide dismutase（SOD）and glutathione（GSH）in gill,liver and muscle tissues of carp were measured.Experimental results indicate that the 24 h-LC50 and 96 h-LC50 concentrations of phenanthrene on carp are 15.926 and11.198 mg/L,respectively;safe concentration is 1.120 mg/L.The significant effect of phenanthrene on carp has been observed during subacute toxicity.An induction effect of SOD has been observed in gill and muscle,and similar trend has been obtained in liver tissue under low concentration of phenanthrene.A suppressed-induced effect has been observed in liver tissue under high concentration of phenanthrene.Similar results were found in GSH.The findings can provide a basis for biomarkers study of PAHs pollution.