下丘脑室旁核apelin对手术创伤大鼠心功能的保护作用（英文）

Apelin in the hypothalamic paraventricular nucleus improves cardiac function in surgical trauma rats

Apelin是一种新型的内源性活性肽。本研究旨在探寻下丘脑室旁核(paraventricular nucleus,PVN)apelin是否能够改善开胸手术创伤大鼠的心功能,以及是否参与了电针的保护作用。将大鼠随机分为正常对照组、开胸手术创伤组和开胸手术创伤+电针内关组,采用荧光定量PCR法检测各组大鼠PVN区apelin及其受体(apelin receptor,APJR)mRNA的表达,然后通过多通道同步记录技术观察PVN微量注射外源性apelin-13(6 mmol/L,0.1μL)对开胸手术创伤组大鼠的血压、心率以及延髓头端腹外侧区(rostral ventrolateral medulla,RVLM)神经元放电活动的影响。结果显示:与正常对照组比较,创伤组大鼠PVN区APJR m RNA的表达显著减少(P<0.05),apelin mRNA的表达也有下降趋势;对开胸手术创伤大鼠施电针双侧内关穴后,PVN区APJR和apelin mRNA的表达水平又有所恢复。PVN微量注射外源性apelin-13可明显升高开胸手术创伤组大鼠的平均动脉压和心率(P<0.05),RVLM神经元单位放电频率也有升高趋势。以上结果提示,PVN区apelin能够改善开胸手术创伤大鼠心功能,也可能参与了电针的保护作用。

Apelin is a novel endogenous active peptide. The aim of this study is to investigate whether apelin in the paraventricular nucleus（PVN） can improve the cardiac function in rats subjected to thoracic surgery trauma, and whether it is involved in the protective effect of electro-acupuncture（EA）. Sprague-Dawley rats were randomly divided into non-stressed group（control）, thoracic surgical trauma stressed group（trauma） and bilateral Neiguan EA applied on thoracic surgical trauma stressed group（trauma ＋ EA-PC 6）. The m RNA expressions of apelin receptor（APJR） and apelin in the PVN were detected by real time-PCR. The exogenous apelin-13（6 mmol/L, 0.1 μL） was microinjected into the rat PVN in the thoracic trauma group, and the effects of apelin-13 on the blood pressure（BP）, heart rate（HR） and the discharge of rostral ventrolateral medulla（RVLM） neurons were observed through the simultaneous recording technology by polygraph. The results showed that the APJR mRNA expression was significantly decreased in the rats of trauma group as compared with that in the control group（P〈0.05）, and a decline trend of apelin mRNA expression was also observed. EA application at bilateral Neiguan acupoints partially recovered the decline of APJR and apelin mRNA expression by the treatment of thoracic trauma. Both mean arterial pressure and HR in the thoracic surgical trauma group were significantly increased by the microinjection of exogenous apelin-13 into the PVN（P〈0.05）, and the single-unit discharge rate of RVLM neurons also had an increasing trend. These results suggest that apelin in the PVN can improve the cardiac function of thoracic surgical trauma rats, and may be involved in the protective effects of EA.