叶酸修饰的CdSe-TiO_2对HL60细胞的光动力灭活作用

Photodynamic Inactivation of HL60 Cells in vitro with Folic Acid-Modified CdSe-TiO_2

研究了叶酸修饰硒化镉掺杂二氧化钛(FA-CdSe-TiO_2)的纳米颗粒体外光动力(PDT)灭活HL60细胞的作用效果,探讨了叶酸修饰增强CdSe-TiO_2纳米颗粒PDT效果的作用机理。利用水解沉积法制备CdSe-TiO_2,采用表面修饰方法制备FA-CdSe-TiO_2纳米颗粒。通过透射电镜(TEM)、傅里叶红外光谱(FTIR)、紫外可见吸收光谱等方法,对纳米颗粒进行结构和光学性质的表征;采用CCK-8法检测细胞活性;利用荧光探针标记技术分析细胞内活性氧水平,通过扫描电镜观察细胞的超微结构。结果表明:FA-CdSe-TiO_2纳米颗粒对细胞暗室毒性相较于CdSe-TiO_2没有大幅变化;而在光照条件下,FA-CdSe-TiO_2对细胞的生长抑制率相比于CdSe-TiO_2有较大提升,其中叶酸比例为1.0时,在18J/cm^2光剂量辐照下,PDT效率达84%;叶酸表面修饰提高了HL60细胞对纳米颗粒的摄取效率,使细胞内活性氧水平提升,进而增强了对HL60细胞的PDT灭活效率。

The vitro photodynamic therapy（PDT）inactivation efficiency of HL60 cells based on cadmium selenidedoped titanium dioxide nanoparticles modified by folic acid（FA-CdSe-TiO_2）is investigated and the mechanism of folic acid modification to enhance the PDT effect of CdSe-TiO_2 nanoparticles is discussed.The CdSe-TiO_2 nanoparticles are prepared by hydrolysis deposition method,and FA-CdSe-TiO_2 nanoparticles are prepared by surface modification method.The structure and optical properties of the nanoparticles are characterized by transmission electron microscopy（TEM）,Fourier transform infrared spectroscopy（FTIR）,ultraviolet visible absorption spectrum and so on.The cell viability is measured by CCK-8 method.The intracellular reactive oxygen species（ROS）levels are analyzed by fluorescence probe labeling technique,and the ultrastructure of the cells is observed by scanning electron microscopy.The results show that FA-CdSe-TiO_2 nanoparticles have no significant change in cytotoxicity compared with CdSe-TiO_2 nanoparticles in the darkroom condition.However,under the light condition,FA-CdSe-TiO_2 has a great increase in cell growth inhibition rate.When the ratio of folic acid is 1.0,the PDT efficiency is 84%at 18 J/cm2 light irradiation.Folic acid surface modification increases the uptake efficiency of HL60 cells to the nanoparticles,and the level of ROS in the cells is increased,thereby the PDT inactivation efficiency of HL60 cells is enhanced.