自制全血质控品用于T淋巴细胞质量控制分析

Self-prepared whole blood samples for quality control of T lymphocyte subset counting

目的评价Cyto-Chex BCT管保存全血标本用于T淋巴细胞亚群计数质量控制的可行性。方法新鲜全血标本在常规CD4检测结束后分别移入Cyto-Chex BCT管混匀,4℃冷藏保存,每隔4 d进行T淋巴细胞亚群计数检测,持续监测56 d,检测数据采用多水平生长模型分析。结果本研究获得了46份全血标本的连续监测结果,其T淋巴细胞亚群CD3、CD4和CD8细胞每次的检测结果与首次检测的结果相关系数均>0.9,除10个结果外,其余检测结果的变异系数均<15%,模型分析计算出平均每天CD3、CD4和CD8细胞各减少约1.46个/μL。结论 CytoChex BCT管保存的全血标本的CD3、CD4、和CD8细胞稳定性良好,可用于CD4检测的室内质量控制和管理,对保证结果的准确性有重要意义。

Objective To evaluate the feasibility of whole blood sample preservation with Cyto-Chex BCT tube for the quality control of T lymphocyte subset counting.Methods The fresh whole blood samples were transferred to Cyto-Chex BCT tubes at the end of the routine CD4 test and stored at 4℃. The T lymphocyte subsets were counted every 4 days continuously for 56 days. Multi-level growth model was adopted in data analysis.Results Totally 46 consecutive samples of whole blood samples were obtained for the study. The correlation coefficients among CD3/CD4/CD8 cell counts at the first test and the each of all consecutive tests were greater than 0.9 and all the coefficients of variation for the cell counts were less than 15% except for only 10 count results. Based on the results of multi-level growth model analysis, the number of CD3/CD4/CD8 count declined with time by 1.46 cells per day averagely.Conclusion Whole blood sample preserved using Cyto-Chex BCT tube can be used in internal quality control and management of CD4 detection because of good stability of CD3/CD4/CD8 cells in the samples under preservation.