一株高效不对称还原产生(R)-苯基乙二醇的菌株筛选、鉴定及全细胞催化体系

Screening and identification of bacterial strain producing(R)-1-phenyl-1,2-ethanediol by efficient whole-cell catalytic reduction system

R）-苯基乙二醇是合成许多光学活性药物的重要手性中间体，其制备具有重要的现实意义. 以α-羟基苯乙酮为底物，从土壤中筛选得到一株能够立体选择性催化α-羟基苯乙酮产生（R）-苯基乙二醇的细菌菌株HBU-SI7. 经形态学观察和16S rDNA序列分析，鉴定此转化菌株为红球菌属菌株Rhodococcus sp. 菌株全细胞催化体系研究表明在邻苯二甲酸二丁酯-磷酸盐缓冲液（V：V = 1：3）的两相催化体系下，菌体转化α-羟基苯乙酮的最优浓度为3.0 g/L，转化率高达96.2%，e.e值为99.3%. 本研究建立的红球菌全细胞催化还原体系对（R）-苯基乙二醇的高效制备具有潜在的研究和应用价值. （图6 表2 参20）

（R）-1-Phenyl-1,2-ethanediol [（R）-PED] is an important chiral intermediate used to synthesize pharmaceutical intermediates. The production of （R）-PED is of great practical significance. A novel bacterial strain HBU-SI7 isolated from soil samples was screened using α-hydroxyacetophenone （2-HAP） as substrate, which is capable of enantioselective reduction of 2-HAP to （R）-PED. HBU-SI7 was identified as Rhodococcus sp. by bacterial morphological observation and 16S rDNA analysis. The analysis of whole-cell catalytic system showed that the bioconversion of 2-HAP reached 96.2% in the two-phase reaction system （dibutyl phthalate/phosphate buffer = 1/3, V/V） with the substrate concentration of 3.0 g/L, and 99.3% of e.e. for （R）-PED was achieved. A new enantioselective reduction system with the whole cells of Rhodococcus sp. was established for efficient production of （R）-PED, which has potential research and application values.