摘要
R)-苯基乙二醇是合成许多光学活性药物的重要手性中间体,其制备具有重要的现实意义. 以α-羟基苯乙酮为底物,从土壤中筛选得到一株能够立体选择性催化α-羟基苯乙酮产生(R)-苯基乙二醇的细菌菌株HBU-SI7. 经形态学观察和16S rDNA序列分析,鉴定此转化菌株为红球菌属菌株Rhodococcus sp. 菌株全细胞催化体系研究表明在邻苯二甲酸二丁酯-磷酸盐缓冲液(V:V = 1:3)的两相催化体系下,菌体转化α-羟基苯乙酮的最优浓度为3.0 g/L,转化率高达96.2%,e.e值为99.3%. 本研究建立的红球菌全细胞催化还原体系对(R)-苯基乙二醇的高效制备具有潜在的研究和应用价值. (图6 表2 参20)
(R)-1-Phenyl-1,2-ethanediol [(R)-PED] is an important chiral intermediate used to synthesize pharmaceutical intermediates. The production of (R)-PED is of great practical significance. A novel bacterial strain HBU-SI7 isolated from soil samples was screened using α-hydroxyacetophenone (2-HAP) as substrate, which is capable of enantioselective reduction of 2-HAP to (R)-PED. HBU-SI7 was identified as Rhodococcus sp. by bacterial morphological observation and 16S rDNA analysis. The analysis of whole-cell catalytic system showed that the bioconversion of 2-HAP reached 96.2% in the two-phase reaction system (dibutyl phthalate/phosphate buffer = 1/3, V/V) with the substrate concentration of 3.0 g/L, and 99.3% of e.e. for (R)-PED was achieved. A new enantioselective reduction system with the whole cells of Rhodococcus sp. was established for efficient production of (R)-PED, which has potential research and application values.
作者
裴朝红
张红蕾
张超
徐志栋
李玮
PEI Chaohong;ZHANG Honglei;ZHANG Chao;XU Zhidong;LI Wei(College of Chemistry & Environmental Science, Hebei University, Baoding 071002, China)
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2018年第2期352-356,共5页
Chinese Journal of Applied and Environmental Biology
基金
河北省高等学校高层次人才科学研究项目(GCC 2014013)
河北大学自然科学研究计划项目(3333112)
河北省自然科学基金专项(B2016201031)资助~~
关键词
红球菌
全细胞催化
不对称还原
α-羟基苯乙酮
(R)-苯基乙二醇
Rhodococcus sp.
whole-cell biocatalyst
asymmetric reduction
α-hydroxyacetophenone
(R)-1-phenyl-1
2-ethanediol