海口地区犬巴贝斯虫病的分子生物学诊断

Molecular biology diagnosis and epidemiological investigation of canines’ Babesia in Hainan

为了研究海南海口地区犬自然感染巴贝斯虫病及虫体DNA基因分型情况,从而为制订海口地区犬巴贝斯虫防疫措施提供依据,试验通过检测贫血犬的血液巴贝斯虫DNA,以海口市流浪狗中心采集的66份犬血样及灵山狗舍采集的23份犬血样（共89份血样）为样本,从中提取基因组DNA并进行PCR扩增,得到18S rRNA基因片段,测序后将所测序列与GenBank中的基因序列进行比较分析,建立系统发育树。结果表明：从89份血液样品中共检测出4个阳性样本,阳性率为4.5%,海口地区犬感染的巴贝斯虫有两个分支,一支属于犬巴贝斯虫,一支属于牛巴贝斯虫。说明了海口地区存在犬巴贝斯虫且同一宿主同一地区的犬巴贝斯虫有不同支系。

To study the natural infection status of Babesia canis and the genotype of Babesia DNA in Hainan Haikou,and provide the basis for the development of the control measures of pests’ Babesia in Hainan province,the blood of anemic dogs and DNA of Babesia from infected dogs were examined. In details,66 samples of dog blood from Haikou City Stray Dog Center and 23 samples of dog blood from Lingshan pet shop（ 89 dog blood samples in total） are collected,the genome DNA was amplified by PCR to obtain the 18 S rRNA gene fragment of Babesia. Then the sequences of amplified gene fragment was compared with the sequences reported in Gen Bank,and the phylogenetic tree was established. The results showed that four positive samples were detected from 89 blood samples with a positive rate of 4. 5%. The Babesia of infected dogs in Haikou province are divided into two branches,one belongs to the Babesia canis,and another belongs to Babesia bovis. The results indicate that the dog Babesia are exist in Haikou area and the same host in the same area of the canine babesiosis have different branches.