摘要
利用尼罗蓝染色法从盐分含量高的垃圾渗滤液处理系统中分离出8株具有PHA合成能力的菌株,通过气相色谱法定量分析PHA产量,进一步筛选出PHA合成能力较强的1株细菌,利用16S rDNA序列分析鉴定菌种,并研究了含盐量和pH对菌株积累PHA特性的影响。结果表明:筛选出的PHA高产菌为蜡样芽胞杆菌(Bacillus cereus)。在盐胁迫下细菌体内的PHA含量提高,在含盐量在5%以内时,盐分对合成PHA产量影响较小,PHA最高产量为61.2 mg/L,最低为57 mg/L,说明该菌有一定的耐盐性。在pH为5~8内,细菌细胞干重变化不大(184.4~199.4 mg/L),最佳pH值为5,此时PHA产量达84.2 mg/L。红外光谱分析表明,利用蜡样芽胞杆菌所得PHA样品具有和标准品相同的化学结构。
Eight kinds of PHA-accumulating strains were isolated from landfill leachate treatment system with high salt content by Nile blue staining. A higher PHA-accumulating strain was screened by quantitative analysis using gas chromatography. The strain was identified by 16S rDNA gene sequencing. The results showed that the high PHA-accumulating strain was Bacillus cereus. Under the stress of salt,the PHA production capacity increased. When the salt content was less than 5%,salt had less effect on PHA yield,which indicated that the strain was salt-tolerant. The dry weight of bacterial cells did not changed obviously(184. 4 ~ 199. 4 mg/L) in a pH range of 5 ~ 8. When the optimal pH was 5,the yield of PHA reached 84. 2 mg/L.The infrared spectroscopic analysis showed that the samples obtained by Bacillus cereus strain had the same structure as the standard products.
作者
王攀
邱银权
陈锡腾
任连海
WANG Pan;QIU Yin-quan;CHEN Xi-teng;REN Lian-hai(School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, China)
出处
《环境工程》
CAS
CSCD
北大核心
2018年第4期78-82,116,共6页
Environmental Engineering
基金
国家自然基金(51578008)
"十二五"国家科技支撑计划(2014BAC27B01-03)
北京工商大学国家两科基金培育项目(LKJJ2016-13)
大学生科研训练计划深化项目(279)
大学生科学研究与创业行动计划项目(201710011100)
"十三五"时期北京市属高校高水平教师队伍建设支持计划(CIT&TCD201804016)