维生素D3对结肠癌增殖和凋亡的影响及机制研究

The effect of vitamin D3 on proliferation and apoptosis of colon cancer

[目的]探讨维生素D3对结肠癌增殖和凋亡的影响及机制研究。[方法]通过对临床结肠癌患者样本收集,血液中维生素D含量检测、受体检测、PCR定量、蛋白质免疫印迹等分子生物学实验,研究维生素D3对结肠癌增殖和凋亡的影响和机制。[结果]ELISA结果显示结肠癌患者血液中的1,25-二羟维生素D3含量(11.54±3.15)ng/ml和2,5二羟维生素D3含量(10.67±2.31)ng/ml较健康对照组1,25-二羟维生素D3为(29.65±7.25)ng/ml,2,5二羟维生素D3为(15.34±6.98)ng/ml明显降低,且维生素D受体的mRNA水平(5.01±3.86)相对对照组(9.36±4.87)较低,免疫荧光技术结果表明维生素D3通过Wnt信号通路影响结肠癌,蛋白质免疫印迹和PCR结果显示维生素D3处理后的人结肠癌细胞SW480中Wnt信号通路相关蛋白,EMT相关蛋白和β-catenin表达量均下调。[结论]维生素D3能够有效减少结肠癌组织的增殖,从而降低结肠癌发病率和防止结肠癌复发。

[Objective]To detect the effect of vitamin D3 on proliferation and apoptosis of colon cancer and its related mechanism.[Methods]Based on clinical patients with colon cancer samples collected,vitamin D levels and its receptor were assayed by ELISA and quantitative PCR.Besides Protein immunoblotting and immunofluorescence technique were employed to study the mechanism of vitamin D3 in regulating the proliferation and apoptosis of colon cancer.[Results]ELISA results showed that vitamin D levels in the blood of the colon cancer patients were significantly lower than that of healthy controls.And the colon cancer patients also showed lower levels of vitamin D receptor at mRNA level.Protein immunoblotting results showed that Wnt signaling pathways related proteins in human colon cancer cells SW480,EMT related proteins andβ-catenin were down-regulated by vitamin D3.[Conclusion]Vitamin D3 can effectively suppress the proliferation of colon cancer tissue,reduce the incidence of colon cancer and prevent the recurrence of colon cancer.