Ⅰ群禽腺病毒4型陕西分离株33K、Fiber-1、Fiber-2、pⅢa基因的克隆及序列分析

Cloning and Sequence Analysis of 33K, Fiber-1, Fiber-2 and pⅢa genes of Fowl Adenovirus Type 4 Isolated from Shaanxi Province

为明确Ⅰ群禽腺病毒陕西分离株相关基因的特征,通过聚合酶链反应(PCR)技术分别扩增了禽腺病毒陕西分离株SX17株的4个基因片段,即33K、Fiber-1、Fiber-2、pⅢa,将其分别克隆到pCold-SUMO载体后进行序列测定,并将克隆得到的4个基因片段与发表的相应序列进行序列比对及遗传进化分析。结果表明,克隆得到的4个片段与Ⅰ群禽腺病毒4型同源性最高,33K基因核苷酸及推导的氨基酸同源性在99.3%~100%与91.5%~100%之间,Fiber-1基因核苷酸及推导的氨基酸同源性在98.5%~100%与65.9%~100%之间,Fiber-2基因核苷酸及推导的氨基酸同源性在98.1%~100%与99.3%~100%之间,pⅢa基因核苷酸及推导的氨基酸同源性在98.6%~100%与98.5%~100%之间。由遗传进化树可知,这些基因在遗传关系上与血清4型位于同一分支,亲缘关系最近,分析显示陕西分离株为Ⅰ群禽腺病毒血清4型。

In order to identify the characteristics of related genes of FAV- I isolated from Shaanxi province, polymerase chain reaction （PCR） was used to amplify the four genes of FAV- I .The gene fragments were then cloned into pCold-SUMO vector and sequenced. The results showed that 33K gene shared 99.3%- 100% nucleotide identity and 91.5%-100% amino acid identity with FAV-I type 4. Fiber-1 gene shared 98.5%-100% nucleotide identity and 65.9%-100% amino acid identity with FAV-I type 4.Fiber-2 gene shared 98.1%-100% nucleotide identity and 99.3%-100%amino acid identity with FAV-I type 4. pill a gene shared 98.6%-100% nucleotide identity and 98.5%-100% amino acid identity with FAV-I type 4. Phylogenetic analysis revealed that these genes seemed to be closely related to serotype 4.