离子色谱法测定盐酸头孢噻呋中的氯离子

Determination of Chloride Ion in Ceftiofur Hydrochloride by Ion Chromatography

建立了离子色谱法测定盐酸头孢噻呋中氯离子含量的方法。试验采用Dionex Ion Pac AS19(250 mm×4 mm,5μm)阴离子交换色谱柱,以氢氧化钾溶液为淋洗液,流速为1.0 m L/min,柱温为30℃,检测池温度为35℃,抑制型电导检测器。试验结果表明氯离子与常见成盐阴离子分离良好,在0.5~20μg/m L浓度范围内呈良好的线性关系(r2=0.9999),加样回收率为101.4%(n=9,RSD=1.7%)。建立的方法准确、灵敏、专属性好,可用于测定盐酸头孢噻呋中氯离子的含量,以控制成盐比例。

The method for content determination of chloride ion in ceftiofur hydrochloride by ion chromatography was developed. Analysis was performed on a Dionex Ion Pac AS19 column（ 250 mm×4 mm,5 μm）. The elutriant was potassium hydroxide solution. The flow rate was 1. 0 m L/min. The column temperature was set at 30 ℃.Inhibitory electrical conductivity detector was used with detecting pool temperature of 35 ℃. The results showed that a good separation was accepted between chloride ion and other conventional anions. The standard curve was linear in the range of 0.5 - 20 μg/m L（ r2= 0.9999）. The average recovery was 101.4%（ n = 9,RSD = 1.7%）. This method was accurate,sensitive,specific and was suitable for the determination of chloride ion content in ceftiofur hydrochloride to control the salt-forming proportion.