HPLC法同时测定银蒲解毒片中4种主要成分的含量

Simultaneous Determination of Four Component in Yinpujiedu Tablets by HPLC

目的建立HPLC法同时测定银蒲解毒片中新绿原酸、绿原酸、隐绿原酸和咖啡酸的含量。方法采用ZORBAX SB-C18色谱柱(250mm×4.6 mm,5μm),以甲醇(A)-0.8%醋酸溶液(B)为流动相,梯度洗脱(0~6 min,15%A→20%A;6~12 min,20%A→30%A;12~15 min,30%A→55%A),流速1.0 m L·min-1,柱温30℃,波长323nm。结果新绿原酸、绿原酸、隐绿原酸和咖啡酸进样量分别在0.108~2.16、0.260~5.20、0.192~3.84、0.024~0.48μg范围内与色谱峰峰面积呈良好线性响应;平均回收率(n=6)分别为99.13%、99.30%、100.59%、101.37%,RSD分别为1.23%、1.12%、1.36%、0.66%。结论该方法对4种成分进行同步测定,准确可靠,重复性好,可用于银蒲解毒片的质量控制。

OBJECTIVE To establish a content determination method of neochlorogenic acid, chlorogenic acid,cryptochlorogenic acid and caffeic acid in Yinpujiedu Tablets simultaneously with HPLC. METHODS Using ZOR- BAX SB-C18 chromatographic column （ 250mm × 4.6mm, 5 μm ）, with mobile phase of methanol （ A ） -0. 8% acetic acid solution （ B）, an eluting gradient （0 - 6rain, 15 % A→20% A ;6 - 12min,20% A→30% A ; 12 - 15 rain, 30% A→ 55 % A） , flow rate of 1.0mL ·min- 1 , column temperature of 30℃ , and wavelength of 323nm. RESULTS Neochlo- rogenic acid, chlorogenic acid ,cryptochlorogenic acid and caffein acid samples weight in the ranges of 0. 108 -2. 16, 0. 260 - 5.20,0. 192 -3.84,0. 024 - 0. 48p, g respectively and linearly corresponds well with the AUC of peaks;average recovery rates （ n = 6） were 99. 13%, 99.30%, 100. 59%, 101.37%, with respective RSD of 1.23%, 1.12% , 1.36% ,0. 66%. CONCLUSION The method determined four components simultaneously with high accu- racy and reproducibility, and can be used for QC of Yinpujiedu Tablets.