喉癌患者的p21WAF1/CIP1和p53表达情况及其临床病理价值研究

Research on the clinical expressions of p21WAF1/CIP1 and p53 and its clinical pathology value for patients with laryngeal cancer

目的探讨喉癌患者的p21WAF1/CIP1和p53表达情况并对其临床病理价值进行研究。方法采用回顾性研究方法,选取2015年1月至2017年1月接收治疗的100例喉癌患者作为研究组,另外选取100例未患有喉癌的急性喉炎患者作为对照组。使用免疫组织化学方法(即S-P法)检测两组患者p21WAF1/CIP1和p53的表达情况,观察研究组p21WAF1/CIP1、p53表达同临床病理参数之间的关系,以及p53蛋白的表达与p21WAF1/CIP1表达的相关性。结果随着分化程度的增加,p21WAF1/CIP1的阳性表达率逐渐增加,高分化患者的阳性表达率明显高于低分化患者(P<0.05);患者p21WAF1/CIP1、p53的阳性表达率与性别、临床分型、T分期以及临床分期之间的差异无显著性(P>0.05);p21WAF1/CIP1在对照组及研究组中的阳性表达率分别为94.0%、64.0%,对照组明显高于研究组,p53在对照组中的阳性表达率为0,研究组为62.0%,研究组明显高于对照组,两组差异有显著性(P<0.05);p53蛋白的表达同p21WAF1/CIP1表达不存在相关性。结论 p21WAF1/CIP1蛋白受到抑制、p53基因表达均与喉癌的发生存在密切的关系,但p21WAF1/CIP1的表达同p53基因的表达之间不存在相关性,明确二者在喉癌患者体内的表达对于临床诊治有重要意义。

Objective To study the clinical expressions of p21WAF1/CIP1 and p53 and its clinical pathology value for patients with laryngeal cancer. Methods 100 patients with laryngeal cancer treated from January 2015 to January 2017 were selected（ study group）. 100 patients without laryngeal cancer were selected（ control group）. The expression of p21WAF1/CIP1 and p53 in the two groups were detected by immunohistochemistry（ S-P method）. The relationship between p21WAF1/CIP1 and p53 expression and clinicopathological parameters in the study group were observed. The correlation between p53 protein expression and p21WAF1/CIP1 expression was analyzed. Results With the higher differentiated degrees,the positive rate of p21WAF1/CIP1 was increased; the positive rate for highly differentiated patients was significantly higher than that of lowly differentiated patients（ P〈0. 05）. The positive rate of p21WAF1/CIP1 and p53 had no correlation with the patient＇s gender,clinical classification,T staging and clinical staging（ P〈0. 05）. The positive rate of p21WAF1/CIP1 for control group（ 94. 0%） was significantly higher than study group（ 64. 0%）. The positive rate of p53 for control group（ 0） was significantly lower than that of study group（ 62. 0%）（ P〈0. 05）. The clinical expressions of p53 had no correlation with the expressions of p21WAF1/CIP1. Conclusion The inhibition of p21WAF1/CIP1 and the p53 gene mutation have a close correlation with the development of laryngeal cancer. However,the clinical expressions of p21WAF1/CIP1 have no correlation with the expressions of p53. The detection of the p21WAF1/CIP1 and p53 is of great importance in the diagnosis of laryngeal cancer.