柚子皮中果胶提取工艺条件优化

Optimization of pomelo peel pectin extraction

目的通过单因素和正交试验优化柚皮中果胶的最佳提取工艺条件。方法在酸水解乙醇沉淀法的基础上,辅助超声波法提取柚子皮中的果胶。以果胶提取率为评价指标,分别研究超声波处理时间、料液比、提取酸度、提取温度对果胶提取率的影响,通过单因素实验筛选出对柚子皮果胶提取率影响显著的因素以及最佳的水平。在单因素分析的基础上进行3因素3水平正交试验研究,最终优化获得柚皮果胶的最佳提取条件。结果柚子皮中果胶提取最佳工艺条件为超声波处理时间为6 min,液料比为8∶1 m L/g,提取p H值为2.00,提取温度为45℃。该提取条件下,柚子皮中果胶得率为23.10%。结论用优化后的工艺提取柚皮中的果胶,提取工艺成本低、时间短、方法简单、条件明确、易操作,适合工业生产。

Objective To optimized the extraction conditions of pectin from pomelo peel by single factor experiment and orthogonal test. Methods On the basis of the acid hydrolysis of ethanol precipitation,auxiliary method of ultrasonic were applied to extract the pectin in the pomelo peel. The effect of ultrasonic processing time,solid-liquid ratio,extract acidity,extraction temperature on extraction rate of pectin were studied respectively,with the extraction rate of the pectin as indicator. The factors that affect the extraction rate and the best level were selected by single factor experiments. 3 factors and 3 levels orthogonal experiments were carried out on the basis of single factor analysis,and the optimum extraction conditions of pectin from pomelo peel were obtained. Results The optimum extraction conditions of pectin from pomelo peel was as follows,ultrasonic processing time was 6 min,liquid-solid ratio was 8∶ 1 m L/g,extract acidity was 2. 00,extraction temperature was 45 ℃. Under the condition of extraction,the yield of pectin from pomelo peel was 23. 10%. Conclusion The optimized extraction process has the advantages of low cost,short extraction time,simple method,definite condition,easy operation and suitable for industrial production.