右心室经胸穿刺测压法在肺动脉高压大鼠模型中的应用

Application of right ventricle pressure by right ventricle punctured through ghest in pulmonary hypertension rats model

探讨应用右心室经胸穿刺测压法作为中期筛查是否能提高肺动脉高压大鼠模型末期成功率。本研究将Sprague Dawley大鼠经胸右心室穿刺法测量右心室压,再实施腹主动脉下腔静脉分流术,术后6周,对实验组大鼠再次测量右心室压,淘汰压力值变化不大的模型大鼠;术后11周,对实验组及对照组进行模型成功率的统计,发现实验组成功率明显高于对照组,结果证明右心室经胸穿刺测压法应用于肺动脉高压大鼠模型的中期筛查可明显提高模型末期的成功率,并可作为一种自身前后对照的新方法。

To investigate whether using the measure of right ventricular puncture by Right Ventricle Punctured through Chest as a metaphase screening can improve the late success rate of pulmonary hypertension in rats. In this study,Sprague Dawley rats were measured right ventricular pressure by transthoracic right ventricular puncture followed by the abdominal aorta-inferior vena cava shunt surgery. After 6 weeks,Rats in the experimental group were re-performed right ventricular pressure measurement to assess,eliminating the rats whose pressure values have a little change compare with before,After 11 weeks,the experimental group and the control group were used to calculate the model success rate,finding that the experimental group is significantly higher than the control group,The results show that transthoracic right ventricular puncture method 6 weeks after can be applied to the medium-term assessment of pulmonary hypertension in rat model,and it can be used as a new method of self-control.