运输应激对肉牛血清中多种应激指标的影响

The effect of transport stress on multiple stress indicators in beef serum

为了研究不同时间运输应激对肉牛血清多种应激指标的影响,试验以15头体重相近且发育良好的肉牛作为研究对象,将其随机分为对照组、运输应激3 h组和运输应激6 h组,每组5头。运输条件为公路运输,时速为80 km/h。运输刺激后,立即采集试验肉牛血液,分离血清。采用酶联免疫吸附试验分别测定血清样品中白细胞激素-1（interleukin-1,IL-1）、白细胞激素-6（interleukin-6,IL-6）、白细胞介素-8（interleukin-8,IL-8）、白细胞介素-10（interleukin-10,IL-10）和肿瘤坏死因子-α（tumor necrosis factor-α,TNF-α）、应激激素皮质醇,以及热休克蛋白70（heat shock protein 70,HSP70）的浓度。结果表明：与对照组相比,皮质醇、IL-6在运输3 h、6 h后均未发生显著性变化（P〉0.05）;在运输应激3 h后,试验肉牛血清中IL-1、IL-8、TNF-α及HSP70浓度未发生显著性变化（P〉0.05）;而在运输应激6 h后,IL-1、IL-8、TNF-α浓度呈显著性水平升高（P〈0.05）,HSP70浓度呈极显著水平升高（P〈0.01）。说明运输3 h未能使肉牛机体产生应激反应,运输6 h可诱发机体产生应激反应。

To study the effect of different duration transports stress on multiple stress indicators in beef serum. Selected 15 beeves that were similar weight and good development,and which were randomly divided into control group）,transport stress 3 h group and transport stress 6 h group,every group had 5 beeves. The transport condition was road transportation and the speed was 80 km/h. After transport stress,blood of the experimental beeves were collected,then to separate serum. Elisa method was used to test the concentration of IL-1,IL-6,IL-8,IL-10,TNF-α,stress hormone cortisol,and stress protein HSP70. Results showed that the concentration of IL-6 and cortisol were no significant difference（P〉0. 05） in test of beeves serum after transport stress 3 h and 6 h which were compared with control group. Compared with control group,the concentration of IL-1,IL-8,TNF-α and HSP70 were no significant difference（P〉0. 05） in test of beeves serum after transport stress 3 h. But after ransport stress 6 h,the concentration of IL-1,IL-8,TNF-α were significant increased（P〈0. 05）,and the concentration of HSP70 was extremely significant increased（P〈0. 01） in serum of test beeves which compared with control group. The conclusion of the experiment showed that 6 h of transport stress could leaded to stress state of experimental beeves,but 3 h of transport stres could not.