Effects and mechanisms of Artemisia argyi essential oil on monocrotaline-induced pulmonary hypertension in rats

艾叶油对肺动脉高压大鼠的干预研究（英文）

In the present study, we aimed to investigate the effects of Artemisia argyi essential oil（AO） on pulmonary hypertension（PH） induced by monocrotaline（MCT） and to explore the underlying mechanism. A total of 80 Sprague-Dawley rats were randomly divided into four groups as follows： control group, model group, bosentan（0.1 g/kg） group and AO（0.1 g/kg） group. After 30 d of experiment, hemodynamic parameters, lung and right ventricle hypertrophy index were determined. HE and Immunohistochemistry staining of lungs were performed to detect the injuries and protein expressions. The results showed that levels of m PAP, m RVP, max RVP, w W, LI, RV and RVHI as well as the expressions of NF-κB p65 and α-SMA were increased, small pulmonary artery thickened, the cavity of the arteriole narrowed, and there was marked infiltration of inflammatory cells in lungs of rats receiving MCT compared with the normal group. After the administration of AO, the levels of m PAP and m RVP were significantly decreased, and the w W, LI, LV＋S and pulmonary arterial remodeling were markedly improved. The expression levels of NF-κB p65 and α-SMA were reduced by AO compared with the model group. Our results suggested that AO reduced the progression of PH induced by MCT through inhibiting the expressions of NF-κB p65 and α-SMA.

本研究建立野百合碱(MCT)诱导的肺动脉高压大鼠动物模型,观察艾叶油对大鼠肺动脉高压的治疗作用。将80只SD大鼠随机分为正对照组、模型组、波生坦组(0.1 g/kg)和艾叶油组(0.1 g/kg)。造模给药30天后,测定血流动力学参数、肺指数(LI)和右心肥厚指数(RVHI)。肺组织切片进行苏木素-伊红(HE)染色,光镜下观察肺组织形态学改变。采用免疫组化法观察大鼠肺组织相关蛋白表达的变化。结果显示,模型组大鼠肺动脉管壁明显增厚,管腔明显狭窄,管周有炎性细胞明显浸润,且模型组大鼠右心肥厚指数(RVHI)及平均肺动脉压(mP AP)较正常对照组明显升高。艾叶油对肺动脉高压大鼠平均肺动脉压、平均右心室压、右心肥厚指数、肺指数和肺动脉的重构均有不同程度的改善;大鼠的左心室加心室间隔重量(LV+S)显著降低。在机制研究中,行野百合碱注射后,NF-κB p65、α-SMA的表达增加,在相同时间段内模型组和药物治疗组差异有统计学意义,表明艾叶油可以显著抑制两者的表达。研究表明艾叶油通过下调肺组织NF-κB p65、α-SMA的表达,抑制肺血管的病变、缓解肺组织损伤、发挥抗肺动脉高压作用。