液相色谱法测定乳饮料中阿司帕坦含量的不确定度分析

Uncertainty evaluationin for the determination of aspartame in drinks containing milk by high performance liquid chromatography

采用高效液相色谱法对乳饮料中阿司帕坦含量的不确定度进行评估。根据JJF 1135—2005《化学分析测量不确定度评定》和JJF 1059.1—2012《测量不确定度评定与表示》中的有关规定,建立测定乳饮料中阿司帕坦含量不确定度的数学模型。根据不确定度的传播规律,逐层分析不确定度来源,对不确定度分量进行量化和合成,计算出相对和扩展不确定度。实验结果表明,乳饮料中阿司帕坦含量为0.118g/kg时,扩展不确定度为0.006g/kg(k=2)。不确定度的最大来源是拟合标准工作曲线产生的不确定度。

According to the relevant regulations presented in Evaluation of Uncertainty in Chemical Analysis Measureament（JJF 1135-2005）and Evaluation and Expression of Uncertainty in Measuraament（JJF 1059.1-2012）,the mathematical model was established.According to the rules of uncertainty transfer,the source of uncertainty that may be introduced were analyzed and each component of uncertainty was quantified and combined.The relative and absolute uncertainty were calculated.Experimental results showed that the content of aspartame was 0.118 g/kg and the relative expanded uncertainty of measurement result of aspartame was 0.006 g/kg（k=2）.The main uncertainty came from the fitting of calibration curve.