空泡型质子泵对阴道微环境的调节及意义

Vacuolar H^+-ATPase regulates vaginal microenvironment

目的探讨空泡型质子泵(VHA)调节阴道微环境的机制。方法选择月经规律期女性18例(对照组),绝经过渡期女性19例(研究组1),绝经期女性16例(研究组2)。取宫颈-阴道部组织(hECE)进行VHA的免疫组化染色及实时荧光定量PCR实验,分析VHA与年龄、血清雌二醇(E2)、阴道pH值等指标之间的相关性。结果 (1)随着三组患者血清E2水平下降,其阴道pH值逐渐升高,VHA的mRNA含量逐渐减少(P<0.01)。(2)三组患者VHA蛋白含量逐渐减少,在生发层基底侧无明显表达。(3)在hECE中VHA的表达与血清E2呈正相关,与年龄、阴道pH值呈负相关(P均<0.01)。结论雌二醇可能通过调节hECE中的VHA改变阴道微环境。

Objective The purpose of this study was to develop the mechanism of vacuolar H^＋- ATPase regulation of vaginal microenvironment. Methods In this research, 53 women were divided into three groups. Their age, serum estradiol （ E2 ） , serum follicle stimulating hormone （ FSH ） , vaginal pH value, and mRNA expression of vacuolar H^＋ -ATPase （VHA） on the ectocervical-vaginal epithelial cells were analyzed. Results （1） As serum E2 levels decreased, the vaginal pH values increased and VHA mRNA declined （P 〈 0. 01 ）. （2） Immunohistochemistry scores decreased in the three groups. VHA expression de-creased in human ectocervical-vaginal epithelial tissues except basal cells. （3） The expression of VHA was positive correlated with estradiol, while negative correlated with age and vaginal pH value （ P 〈 0. 01 ）. Conclusions Estradiol could regulate the genetic transcription and synthetic of VHA protein under vaginal microenvironment.