柚皮素通过ROS/JNK/Bcl2通路抑制宫颈癌Hela细胞增殖和迁移

Naringenin inhibits cervical cancer Hela cell proliferation and migration via the ROS/JNK/Bcl2 signaling pathway

目的:探讨柚皮素对宫颈癌Hela细胞增殖和迁移影响及分子机制。方法:应用MTT法检测柚皮素对宫颈癌Hela细胞活力的影响,Transwell检测柚皮素对Hela细胞迁移的影响,DCFH染色检测柚皮素对宫颈癌Hela细胞活性氧(ROS)生成的影响,流式细胞仪检测柚皮素对宫颈癌Hela细胞凋亡的影响,Western blot法检测柚皮素对细胞内p-JNK和Blc2蛋白表达的影响。结果:12.5、25、50、100、200μg/ml柚皮素处理细胞24h,细胞活力呈浓度依赖性降低。ROS抑制剂(NAC)能显著抑制柚皮素对细胞活力的抑制作用。50μg/ml柚皮素处理细胞24h能抑制细胞迁移、促进细胞ROS生成和细胞凋亡,细胞凋亡率为(42.2±1.6)%;促进p-JNK的表达,抑制Bcl2的表达,p-JNK表达水平为(2.34±0.12)倍,Bcl2表达水平为(0.22±0.03)倍。结论:柚皮素可诱导宫颈癌Hela细胞ROS的产生,通过激活ROS/JNK/Bcl2通路诱导细胞凋亡,抑制细胞增殖和迁移。

Objective： To investigate the molecular mechanism of Naringenin inhibiting the proliferation of cervical cancer. Methods： The influence on the viability of cervical cancer was evaluated by MTT assay. The effect of Naringenin on migration of Hela was detected by Transwell.Intracellular reactive oxygen species（ROS） generation and apoptosis induced by Naringenin were measured by flow cytometry analysis. Protein levels of JNK,p-JNK and Bcl2 were examined by Western blot analysis. Results： 12. 5,25,50,100,200 μg/ml Naringenin significantly inhibited the viability of Hela in a dose-dependent manner. The inhibition of Naringenin on cell viability was suppressed by the ROS scavenger NAC. The ROS generation,apoptosis and phosphorylation of JNK were induced by Naringenin at 50 μg/ml after 24 hours＇ treatment. The apoptosis rate was（42. 2 1. 6） %. The phosphorylation level of JNK increased（2. 34 0. 12） times. The expression of Bcl2 increased（0. 22 0. 03） times. The expression of p-JNK and Bcl2 and apoptosis induced by Naringenin were suppressed by NAC and SP600125. Conclusion： Naringenin inhibits cervical cancer proliferation via the ROS/JNK/Bcl2 signaling pathway.