摘要
目的在不同温度(10、20、30、37℃)下,比较17株不同耐药性、耐药表型及基因型不一致的副溶血性弧菌在碱性蛋白胨水(3%NaCl)中最大比生长速率及生长快慢之间的差异。方法应用Bioscreen C全自动微生物生长曲线分析仪测定副溶血性弧菌的最大比生长速率,构建不同温度下生长曲线,采用修正后的Gompertz模型进行拟合得出生长动力学参数。结果不同耐药性副溶血性弧菌μmax存在一定差异,差异随着温度的降低而增加,抗生素耐药种类越多,其μmax越大;同一温度下,耐药表型与基因型不一致的副溶血性弧菌其生长参数存在一定的差异,但是菌株的生长参数的差异与菌株耐药表型与基因型不一致之间没有明显的对应关系。结论菌株之间的微生物生长动力学参数的异质性将会对风险评估的准确性带来影响,此结果可为多重耐药副溶血性弧菌风险评估提供基础数据支持。
Objective To compare the maximal specific growth rate and growth rate of 17 strains of Vibrio parahaemolyticus with different drug resistance, drug-resistant phenotype and genotype incongruent in alkaline peptone water(APW, 3%NaCl) at different temperatures(10, 20, 30, 37 ℃). Methods The maximum specific growth rate of Vibrio parahaemolyticus was determined by Bioscreen C automatic microorganism growth curve analyzer. The growth curves of different temperatures were constructed and the growth kinetic parameters were obtained by using the modified Gompertz model. Results There was a certain difference between different drug resistant Vibrio parahaemolyticus, and the difference increased with the decrease of temperature. The more variety of antibiotic resistance, the bigger the μmax was. At the same temperature, there was a certain difference in the growth parameters between the resistant phenotype and the genotype incongruent Vibrio parahaemolyticus. However, there was no significant correlation between the growth parameters of the strains and the inconsistency of the resistant phenotype and genotype of the strain. Conclusion The heterogeneity of microbial growth kinetics parameters between strains will affect the accuracy of risk assessment. This result can provide basic data support for the risk assessment of multi-drug resistant Vibrio parahaemolyticus.
作者
牛丽
巴永兵
白凤佳
潘迎捷
赵勇
刘海泉
NIU Li;BA Yong-Bing;BAI Feng-Jia;PAN Ying-Jie;ZHAO Yong;LIU Hai-Quan(College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China;Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China;Laboratory of Quality & Safety Risk Assessment for Aquatic Product on Storage and Preservation (Shanghai), Ministry of Agriculture Shanghai 201306, China;Engineering Research Center of Food Thermal-processing Technology, Shanghai Ocean University, Shanghai 201306, China)
出处
《食品安全质量检测学报》
CAS
2018年第8期1802-1809,共8页
Journal of Food Safety and Quality
基金
国家自然科学基金项目(31571917
31671779)
上海市科技兴农项目(沪农科攻字2015第4-8号
农科攻字2016第1-1号
沪农科推字2017第4-4号)
上海市教育委员会科研创新计划资助
上海市教委曙光计划(15SG48)~~
关键词
耐药
副溶血性弧菌
最大比生长速率
生长异质性
drug resistant
Vibrio parahaemolyticus
maximum specific growth rate
growth heterogeneity