沙蚕多肽提取及功能研究

Extraction and function of polypeptides from Nereis diversicolor

目的通过研究沙蚕酶解及其多肽产物的功能,提高沙蚕的利用率。方法采用碱性蛋白酶、胃蛋白酶、中性蛋白酶对沙蚕进行酶解,以水解度为评价指标,比较3种酶解产物的抗氧化活性、总还原力能力和降血糖功能。结果 3种酶中中性蛋白酶在6 h达到最高水解度37.8%。碱性蛋白酶酶解7 h的产物其亚铁离子螯合力最高,可达32.7%;DPPH自由基清除能力最高可达85.8%,为中性蛋白酶酶解6 h的产物;胃蛋白酶酶解6 h产物的总还原力最高;胃蛋白酶酶解3 h的酶解产物其DPP-4抑制率最高,可达52.9%。结论胃蛋白酶更适于沙蚕的酶解。酶解产物具有较理想的抗氧化及降血糖功能,为沙蚕多肽产品的开发奠定基础。

Objective To improve the utilization rate of Nereis diversicolor by studying the function of enzymatic hydrolysis and polypeptide products. Methods The enzymatic hydrolysis of silkworm by alkaline protease, pepsin and neutral protease was studied. The antioxidation, total reduction and hypoglycemic function of three enzymolysis products were compared with hydrolysis evaluation index. Results Neutral protease reached the highest hydrolysis degree of 37.8% in 6 h among the three enzymes. The product of alkaline protease enzymatic hydrolysis for 7 h had the highest chelating power of ferrous ion, which could reach 32.7%. The scavenging ability of DPPH free radical was up to 85.8%, which was the product of neutral protease hydrolysis for 6 h. The total reduction ability of pepsin enzymatic hydrolysis product was the highest at 6 h, and the DPP-4 inhibition rate of pepsin hydrolyzed for 3 h was the highest, up to 52.9%. Conclusion Pepsin is more suitable for enzymatic hydrolysis of Nereis diversicolor. The enzymatic hydrolysis products have better antioxidation and hypoglycemic functions, which can lay a foundation for the development of Nereis diversicolor polypeptides.