脆性组氨酸三联体基因表达在外阴癌诊断中的作用

The Role of Fragile Histidine Triad Gene Expression in the Diagnosis of Vulvar Carcinoma

目的探索脆性组氨酸三联体基因表达在外阴癌诊断中的作用。方法采用的方法为免疫组化SP法,分别对正常外阴组织25例、外阴上皮内瘤样病变(VINs)29例和外阴癌组织40例实施FHIT蛋白表达的检查。结果正常外阴组织、VINs、外阴癌FHIT蛋白阳性表达率分别为100.0%(25/25),44.8%(13/29),20.0%(8/40),差异有统计学意义(P<0.05)。有淋巴结转移FHIT蛋白阳性表达率为18.2%(2/11),明显低于无淋巴结转移者的27.6%(8/29),差异有统计学意义(P<0.05)。结论 FHIT蛋白的异常表达为诊断外阴癌提供了有力的依据,对外阴癌的治疗与预后提供重要的信息。

Objective To explore the role of fragile histidine triad gene expression in the diagnosis of vulvar carcinoma. MethodsThe method of immunohistochemistry SP was used. The expression of FHIT protein was examined respectively in 25 cases of normal vulvar tissue, 29 cases of intraepithelial neoplasia（VINs） and 40 cases of vulvar carcinoma. Results The positive expression rates of FHIT protein in normal vulvar tissue, VINs and vulvar carcinoma were respectively 100.0%（25/25）, 44.8%（13/29）, 20.0%（8/40）,the difference was statistically significant（P〈0.05）. The positive expression rate of FHIT protein with lymph node metastasis was 18.2%（2/11）, which was significantly lower than that of those without lymph node metastasis [27.6%（8/29）], and the difference was statistically significant（P〈0.05）. Conclusion The abnormal expression of FHIT protein provides a strong basis for the diagnosis of vulva cancer, and provides important information for the treatment and prognosis of external cancer.