荧光成像系统对比度分析与成像仿真

Contrast analysis and imaging simulation of fluorescence imaging system

目前荧光成像技术在生物医学领域得到越来越广泛的应用。为了缩短产品设计和开发周期,且能更直观地反映系统的成像效果,根据各模块光谱特性曲线,提出了荧光成像链路模型。利用该模型,对荧光成像系统的对比度进行了分析,验证了滤光片光密度(OD)值的合理范围是在5~7之间。最后以荧光显微镜为例,对系统成像过程进行了仿真。结果表明,各模块不匹配也会对系统对比度产生影响,仿真图像能够直观反映出系统的匹配程度和成像效果,并与实际系统测试结果相吻合,证明了该链路模型仿真的可行性和有效性。

Currently,the fluorescence imaging system is widely used in the biomedical fields.In order to reduce fluorescence product development cycles and to show the visual effects of imaging system,the model of fluorescence imaging chain is proposed by using the spectral characteristic curves of each module.According to the chain model,the imaging system contrast is analyzed.The results prove that the recommended range of filtered optical density（OD）should be 5-7.Finally,the imaging process of the system is simulated by the fluorescence microscopy.The simulated images show the image quality and the match those obtained from the actual system.It proved that the model of fluorescence imaging chain is feasible and effective.