过敏性鼻炎患者肺虚感寒证和肾阳亏虚证血清代谢组学研究

Study on serum metabolomics of lung deficiency and cold syndrome and kidney-yang deficiency syndrome in patients with allergic rhinitis

[目的]基于气相色谱/质谱(GC/MS)技术的代谢组学方法,分析过敏性鼻炎肺虚感寒证和肾阳亏虚证患者血清中的内源性小分子代谢物变化,为两种中医证型的科学辨证提供依据。[方法]采用GC/MS技术检测过敏性鼻炎患者肺虚感寒证、肾阳亏虚证以及正常人的血清中小分子代谢物,利用SIMCA-P软件进行主成分分析(PCA)及偏最小二乘判别法(PLSA-DA)分析,研究血清中小分子代谢物在各组间的差异。[结果]过敏性鼻炎肺虚感寒证、肾阳亏虚证患者代谢谱具有良好的分离趋势,并与健康人对照组有明显区分。利用商业化的代谢物谱库(如Wiley和NIST质谱库)及本实验室建立的标准品代谢物谱库鉴定了23个具有统计学意义的差异代谢物。在过敏性鼻炎肺虚感寒证和肾阳亏虚证患者中筛选得到了丙氨酸、十四烷酸、十六烷酸、9,12-十八碳二烯酸、9-(Z)-O-十八碳烯酸、十八烷酸6种差异代谢产物。[结论]过敏性鼻炎肺虚感寒证和肾阳亏虚证的代谢差异主要体现在氨基酸代谢和脂肪酸代谢方面,基于GC/MS的代谢组学技术可用于临床不同中医证型的分类研究。

[Objective] Based on GC/MS metabolomics techniques, endogenoussmall molecule metabolitesof serum onlung deficiency and cold syndrome and kidney-yang deficiency syndrome in patients with allergic rhinitiswere analyzed, and thereby providing scientific evidence for syndrome typing of them. [Methods] Small molecule metabolites in the serum of allergic rhinitis with lung deficiency and cold syndrome and kidney-yang deficiency syndrome as well as normal control group were detected with GC/MS. PCA and PLS-DA were analyzed using SIMCA-P software, differences of small molecule metabolites inserum among the groups was studied. [Results] Metabolic profile appeared good separation trend between allergic rhinitis with lung deficiency and cold syndrome and kidney-yang deficiency syndrome, three groups were distinguished clearly. The 23 statistically significant differences metabolites were identified using commercially available metabolite libraries（such as the Wiley and NIST mass pools） and the standard metabolite library established in our laboratory. Four differentially identified metabolites were screened from serum of allergic rhinitis with lung deficiency and cold syndrome and kidney-yang deficiency syndrome included alanine, tetradecanoic acid, hexadecanoic acid, 9, 12-octadecadienoic acid, 9-（Z）-O-octadecenoic acid, octadecanoic acid. [Conclusion] The metabolic differences of allergic rhinitis with lung deficiency and cold syndrome and kidney-yang deficiency syndromemainly manifested in amino acid metabolism and nucleotide metabolism,and based on GC/MS metabolomics techniquesmay be used in classified study of Chinese medical syndrome typing.