甜瓜种子无菌萌发条件优化

Optimization of Cucumis melo L.Seed Sterile Germination Conditions

为优化甜瓜无菌萌发条件,采用正交设计L_9(3~4)研究了甜瓜种子在不同培养基(琼脂培养基、1/2 MS和1/8 MS培养基)、不同浸种时间(0.5、2.0、4.0h)以及培养温度(25、30、35℃)下发芽情况。结果表明:甜瓜浸种4h,浸种温度是35℃,接种在1/2 MS培养基,25℃的培养温度条件下进行培养,甜瓜种子发芽率达到100%。

In order to optimize Cucumis melo L.seed sterile germination conditions,we compared the germination in different medium（agar medium,1/2 MS,1/8 MS）,different seed soaking time（0.5,2.0,4.0 h）and culture temperature（25,30 and 35℃）by the orthogonal design L 9（3 4）.The results showed that seed soaking temperature at 35 ℃,seed soaking time for 4 h,culture temperature at 25 ℃,the 1/2 MS,sterile germination rate was the best germination condition,the germination rate reached 100%.