摘要
为评价两种不同流动相的可行性,通过测定3种蛹虫草中的虫草素与腺苷的含量,对比高效液相色谱(HPLC)的甲醇-水和乙腈-水两种不同流动相。结果表明:HPLC法的两种不同流动相测定虫草素含量的保留时间分别为6.6和15.0min;测定腺苷含量的的保留时间分别为5.7和11.9min。其中,以甲醇-水为流动相所测得的虫草素含量更高,在检测时更为便捷,保留时间也较适合。
In order to evaluate the feasibility of two different mobile phases,the content of cordycepin and adenosine in 3 species cordyceps militaris was determined by comparing 2 different mobile phases of methanol water and acetonitrile water of high performance liquid chromatography（HPLC）.The results showed that the retention time of cordycepin under the 2 different mobile phases in the HPLC method was 6.6 and 15 min,respectively;the retention time for the determination of adenosine content was 5.7 and 11.9 min,respectively.Among them,the content of cordycepin with methanol-water as the mobile phase was higher,and it was more convenient in the detection and the retention time was more suitable.
作者
张鑫喆
赵琳琳
徐方旭
柳叶飞
王升厚
ZHANG Xin-zhe;ZHAO Lin-lin;XU Fang-xu;LIU Ye-fei;WANG Sheng-hou(College of Life Sciences,Shenyang Normal University,Shenyang 110034 ,China;Exper imental Teaching Center of Shenyang Normal University,Shenyang 110034,China)
出处
《黑龙江农业科学》
2018年第4期130-132,共3页
Heilongjiang Agricultural Sciences
关键词
高效液相色谱
流动相
虫草素
腺苷
high performance liquid chromatography（HPLC）
mobile phase
cordycepin
adenosine
