摘要
基于3种雌激素(雌二醇、雌三醇和炔雌醇)相似的荧光性质,建立起荧光法直接快速测定奶粉中3种雌激素的总量。以雌三醇为标准参考物质,采用标准曲线法测定。雌三醇的荧光强度与浓度在1~2000 nmol/L的范围内呈线性关系,方法的检出限为0.16 nmol/L,回收率在89.11%~98.49%之间。该方法简单、快速、线性范围宽、准确度和灵敏度高。用于实际奶粉中3种雌激素总量的测定,结果满意。
Based on the similarity of the fluorescence properties of three estrogens,a new fluorescence method for direct determination of the total amount of three estrogens（ estradiol,estriol,ethinyloestradiol） in milk powder was developed. Taking estriol as the standard reference material,the standard curve method was adopted. The fluorescence intensity of estriol has a linear relationship with the concentration in the range of1. 00 ～ 2000 nmol/L and the detection limit of 0. 16 nmol/L. The recovery is in the range of 89. 11% ～98. 49%. This method is simple,rapid,highly accurate and sensitive and has been successfully applied to determine the total estrogens in milk powder with satisfactory results.
作者
蔡其洪
陈小萍
鲁家兴
陈登秀
CAI Qihong;CHEN Xiaoping;LU Jiaxing;CHEN Dengxiu(Key Laboratory of Pharmaceutical Analysis and Laboratory Medicine of Fujian Province University, Pharmaceutical and Medical Technology College, Putian University, Putian , Fujian 351100, China)
出处
《应用化学》
CAS
CSCD
北大核心
2018年第5期609-612,共4页
Chinese Journal of Applied Chemistry
基金
福建省科技计划引导性项目(2015Y0030)
福建省教育厅科技项目(JK2014045)
莆田市科技计划项目[2013S01(2)]
莆田学院科研项目(2015088)
关键词
雌激素
荧光分析法
奶粉
雌二醇
雌三醇
炔雌醇
estrogens
fluorescence spectrometry
milk powder
estradiol
estriol
ethinyloestradiol
