水稻黄绿叶基因Yellow-Green Leaf 6(YGL6)的表达模式与蛋白定位

Expression Pattern and Protein Localization of a Yellow-Green Leaf 6(YGL6) Gene in Rice(Oryza sativa)

叶色突变既可作为形态标记用于杂交稻育种,又是研究光合系统的结构和功能、叶绿素生物合成及其调控机制的理想材料。EMS(ethyl methane sulfonate)诱变籼稻恢复系"缙恢10号"获得1个稳定遗传的黄绿叶突变体,暂命名为ygl6(yellow-green leaf 6)。前期我们通过图位克隆筛选出候选基因Os12g23180,通过遗传互补实验证实了黄绿叶基因YGL6为Os12g23180,BLASTp分析表明YGL6基因编码NAD(P)-结合的Rossmann折叠超家族蛋白质,属于短链脱氢酶/还原酶家族,推断为异黄酮还原酶、糖脱水酶或mRNA结合蛋白。利用qRT-PCR进行表达模式的分析表明YGL6基因仅在绿色组织如心叶、成熟叶、叶鞘和绿色颖壳中表达,尤其以心叶的表达量最高,同时YGL6基因表达还受光照的诱导。构建亚细胞定位载体,转水稻原生质体结果表明YGL6蛋白定位于叶绿体。本研究丰富了水稻突变体库,为YGL6基因的功能分析奠定了基础。

Leaf color mutants are used not only as morphological markers in hybrid rice breeding, but also as ideal materials in studies on the structure and function of photosystem, chlorophyll biosynthesis and regulation mechanism. A new rice mutant exhibiting stable inheritance was derived from ethyl methane sulfonate （EMS）-treated restorer line Jinhui 10 （Oryza sativa）, tentatively named as yellow-green leaf 6 （ygl6）. The ygl6 leaf displayed yellow-green at seeding stage, and pale green at jointing stages. The YGL6 complementation experiment implied that the Os12g23180 is the YGL6 gene. The expression pattern analysis indicated that YGL6 was expressed in green tissues including young leaves, mature leaves, sheaths and green glume, with the highest expression level in young leaves. And YGL6 expression was induced by light. Transient expression of the YGL6-GFP protein in rice protoplast showed that YGL6 was localized in chloroplasts. These results provide a foundation for functional analysis of YGL6.