摘要
目的探究甲酰肽受体1在膀胱癌细胞中的表达及其激活后的作用。方法利用实时荧光定量PCR方法和蛋白免疫印迹法检测正常膀胱上皮细胞和T24和pumc-91细胞甲酰肽受体1mRNA和蛋白的表达量,用甲酰肽受体1的激活剂f MLP激活甲酰肽受体1后,实时荧光定量PCR方法检测IL-8 mRNA表达量。结果基因水平上,甲酰肽受体1在两株膀胱癌细胞和正常的膀胱上皮细胞中均表达,与正常的膀胱上皮细胞相比T24和pumc-91细胞均低表达;蛋白水平上,FPR1在两株膀胱癌细胞中也均表达,与正常膀胱上皮相比T24和pumc-91细胞均高表达。f MLP激活FPR1后,T24和pumc-91膀胱癌细胞IL-8mRNA的表达量增加。结论 FPR1在膀胱癌细胞中高表达并且调控T24和pumc-91细胞IL-8mRNA的分泌,可能与膀胱癌的发生发展相关。
Objective This study was to explore the differential expression and functional role of Formyl Peptide Receptor1 in bladder cancer. Methods The expressions of FPR1 in T24 and pumc-91 cells were measured by qRT-PCR and Western Blot. T24 and pumc-91 cells were cultured with fMLP for 48 h,and then IL-8 mRNA was measured by qRT-PCR. Results FPR1 gene had low expression,while FPR1 protein was over expressed in T24 and pumc-91 bladder cancer cells compared with SV-HUC-1 normal bladder epithelial cells. FPR1 activation improved the expression of IL-8 mRNA. Conclusion FPR1 is highly expressed in bladder cancer cells and regulates the secretion of IL-8 mRNA in T24 and pumc-91 cells,which may be related with the development and progression of bladder cancer.
作者
姜雪
张曼
JIANG Xue;ZHANG Man(Department of Clinical Laboratory, Capital Medical University, Beijing Shijitan Hospital, Department of Clinical Laboratory, Peking University Ninth School of Clinical Medicine, Beijing Key Laboratory of Urinary Cellular and Molecular Diagnostics, Beijing 100038 ,China)
出处
《标记免疫分析与临床》
CAS
2018年第4期565-568,583,共5页
Labeled Immunoassays and Clinical Medicine
基金
北京市自然科学基金(编号:7172106)
北京市医管局首届登峰人才计划(编号:DFL20150701)
