二肽基肽酶9基因单核苷酸多态性与煤工尘肺的相关性

Correlation between single nucleotide polymorphisms of DPP9 gene and coal workers' pneumoconiosis

目的探讨二肽基肽酶9基因(DPP9)rs12610495位点单核苷酸多态性(SNP)与唐山地区汉族人群煤工尘肺(CWP)的相关性。方法采用基质辅助激光解吸电离子飞行时间质谱技术(MALDI-TOF MS)对652例CWP患者和648例接尘健康对照者DPP9基因的rs12610495位点分型,采用PLink 1.07软件对SNP位点基因型和等位基因频率及遗传模型进行分析。结果与对照组比较,CWP组DPP9基因rs12610495位点的基因型和等位基因频率均无明显变化,差异无统计学意义(χ~2=1.731、0.565,P>0.05)。遗传模型分析显示,DPP9基因rs12610495位点在相加、显性及隐性三种遗传模型下的基因型分布比较,差异均无统计学意义(OR=1.087、0.987、1.964,95%CI=0.870~1.358、0.659~1.478、0.527~7.314,P均>0.05)。结论 DPP9基因rs12610495位点单核苷酸多态性可能与唐山地区汉族人群CWP的易感性无关。

Objective To investgate the association between single nucleotide polymorphism （SNP） of dipeptidyl-peptidase 9 （DPPg） rs12610495 loci and coal workers＇ pneumoconiosis （CWP） of Hun nationality in Taugshan area. Methods The geno- typing for SNP rs12610495 loci of DPP9 gene from 652 CWP patients （CWP group） and 648 dust-exposed workers （control group） were made using matrix associated laser desorption/ionization-time of flight mass spectrometry （MALDI-TOF MS）, the genotype, allele frequency and genetic model were analyzed with PLink 1.07 software. Results There was no significant differ- ence found in genotype and allele frequency of DPP9 gene rs12610495 between CWP group and control group （X2 = 1. 731 and 0. 565 respectively, both P〉0. 05）. The genetic model test also showed that in genotypes distribution comparisons none of any three genetic models of additive, dominant and recessive showed significant difference with controls （ OR = 1. 087, 0. 987 and 1. 964 respectively, all P〉0.05）. Conclusion The results suggested that SNP of DPP9 gene rs12610495 loci may not be asso- ciated with CWP susceptibility in Han workers of Tangshan area.