海洋来源真菌杂色曲霉的化学成分及抗肿瘤活性研究

Study on chemical constituent and antitumor activity of a marine-derived fungus Aspergullus versicolor

目的研究1株海洋来源真菌Aspergullus versicolor NM-021杂色曲霉的化学成分及其抗肿瘤活性。方法利用硅胶柱色谱、Sephadex LH-20凝胶柱色谱、半制备HPLC等色谱技术对其化学成分进行分离、纯化;通过NMR、MS方法,结合与文献报道的数据比对,对化合物的结构进行鉴定。运用细胞毒活性筛选模型对其抗肿瘤活性进行初步评价。结果从该菌株中分离得到了2个蒽醌类化合物2 H-6-O-Methylaverufin(1)和6-O-Methylaverufin(2)。生物活性测试结果表明,化合物1对人结肠癌和食管癌具有一定的抑制活性。结论从该菌株中分离得到1个新的具有细胞增殖抑制活性的蒽醌类化合物(1)。

Objective To study the chemical constituent and antitumor activity of a marine-derived fungus Aspergullus versicolor NM-021.Methods The compounds were purified by silica gel and Sephadex LH-20 column chromatography,followed by semi-preparative HPLC.Their structures were identified by NMR and MS analysis as well as comparison with literatures.Results Two anthraquinones,2 H-6-O-Methylaverufin（1）and 6-OMethylaverufin（2）were isolated from this marine-derived fungus Aspergullus versicolor.Compound1 showed moderate cytotoxic effects against the cell line of colon and esophageal cancer.Conclusion A novel anthraquinone（1）with cytotoxic activity was isolated from this fungus.