小脑变性相关蛋白1反义转录物的研究进展

The regulatory role of cerebellar degeneration-related protein 1 antisense

环状RNA是一类缺乏5′末端帽子和3′末端多聚核苷酸尾共价结合的单链RNA环状分子。作为非编码RNA的新成员，环状RNA较其他线性RNA分子具有不同物种间更保守、不易被RNA酶降解、不同组织间特异分布且含量较高等优点。因此，环状RNA替代其他线性RNA成为新的研究热点。作为目前研究较多的环状RNA分子，小脑变性相关蛋白1反义转录物（CDR1as）拥有74位微小RNA-7结合位点；其不仅可通过竞争性内源RNA途径阻碍微小RNA-7功能还可以大量储存微小RNA-7并适时释放。CDR1as可稳定存在于血浆外泌体中并与诸多疾病预后指标相关。

Circular RNA is a kind of single-stranded RNA ring without 5′capping and 3′polyadenylation. As a new member of non-coding RNA, circular RNA is evolutionally conserved, relatively stable, highly specific and enormously abundant. Instead of liner RNA, circular RNA became the research hotspot. So far, cerebellar degeneration-related protein 1 antisense （CDR1as） is a molecule that has garnered intense research. CDR1as harbors 74 conventional miRNA-7 binding sites. Expression of CDR1as efficiently tethers micro RNA-7, resulting in reduced micro RNA-7 activity and increased levels of micro RNA-7 targeted transcripts consistent with the micro RNA sponge and competing endogenous RNA （ceRNA） hypothesis. Moreover, CDR1as can restore micro RNA-7 and release it at right time. CDR1as can be stabilized in the exosomes. And its level has correlation with many clinical characteristics.