基于几何形态学的三种库蠓翅的形态变化分析

Geometric morphometric analysis of wing shape variation among three important species of the genus Culicoides (Diptera: Ceratopogonidae)

【目的】基于几何形态学方法定量分析库蠓亚属中3个近似种(刺螫库蠓Culicoides punctatus、灰黑库蠓Culicoides pulicaris和新替库蠓Culicoides newsteadi)翅的形态变化规律,以期进行准确鉴定。【方法】运用几何形态学标点(Landmark)对3种库蠓的翅进行量化分析,并结合主成分分析法(Principal component analysis,PCA)、典型变量分析法(Canonical variate analysis,CVA)、薄片样条(Thin-plate spline,TPS)及聚类分析法对3种库蠓翅进行分析比较,探讨其形态变异。【结果】3种库蠓的翅在翅型、翅脉结构上存在着显著差异,主要发生在径中横脉、径室区,以及翅基部和端部。【结论】几何形态学能够作为一种工具对蠓科昆虫进行种间关系及分类研究。

[Objectives] To assess geometric morphometrie analysis of wing shape as a means of accurately identifying three biting midges of the genus Culicoides Latreille, C. punctatus, C. pulicaris and C. newsteadi. [Methods] Various geometric methods were used to analyze wing shape variation, including landmarks, principal component analysis, canonical variate analysis, thin-plate splines and cluster analysis. [Results] Significant differences in wing shape and venation were apparent among the three species, the main differences were in the radio-median cross-vein, radial cell, and basal and distal portions of the wing. C. punctatus and C. newsteadi were most similar in wing shape and venation. [Conclusion] Geometric morphometrics is a useful tool for identifying interspecific relationships within the Ceratopogonidae.