miR-19a与TNF-α在口腔扁平苔藓患者中的表达情况及相关性

Expression and correlation of miR-19a and TNF-α in patient with oral lichen planus

目的探究口腔扁平苔藓(OLP)患者miR-19a与TNF-α的表达情况,分析二者与临床病理参数的关系,并分析二者的相关性。方法 2015年1月—2016年1月宁波市第二医院经病理诊断为OLP患者的口腔黏膜组织标本43例作为研究组,另取同期口腔鳞状细胞癌组织标本30例和体检健康者正常口腔组织标本30例作为对照组,应用实时荧光定量PCR(qRT-PCR)检测组织中miR-19a与TNF-αmRNA的表达情况,并分析其与口腔扁平苔藓患者临床参数的关系。结果与正常口腔黏膜组相比,口腔扁平苔藓组中miR-19a(t=5.912,P<0.001)和TNF-αmRNA(t=21.938,P<0.001)表达水平显著增加。与口腔鳞状细胞癌组相比,口腔扁平苔藓组miR-19a(t=3.980,P<0.001)和TNF-αmRNA(t=6.052,P<0.001)表达水平显著降低。miR-19a和TNF-α表达水平与患者年龄、性别、病程、临床表型有关(P<0.05)。在口腔扁平苔藓组织中随miR-19a表达水平的增加,TNF-αmRNA表达水平逐渐增加,二者呈正相关(r=0.495,P<0.05)。结论 OLP患者组织中miR-19a与TNF-αmRNA表达水平显著增加,与患者年龄、性别、病程及临床表型有关,且二者呈正相关。

Objective To investigate the expression of miR-19a and TNF-α in patients with Oral lichen planus （ OLP）, explore the relation to each other, and analyze the relationship between them and clinicopathological parameters. Meth- otis From January, 2015 to January, 2016, 43 cases of oral mucosal tissue from OLP patients （study group）, 30 cases of oral squamous cell carcinoma specimens （carcinoma gorup ） and 30 cases of normal oral tissue samples （control group ） were collected. The expression of miR-19a and TNF-α mRNA in different tissues from the three groups was detected by real-time quantitative PCR （qRT-PCR） , and the relationship between the expression of miR-19a and TNF-α mRNA and the clinical parameters of patients with oral lichen planus was analyzed. Results Compared with the control group, the expression levels of miR-19a（t = 5. 912, P 〈 0. 001 ） and TNF-α mRNA （t = 21. 938, P 〈 0. 001 ） in the study group were significantly increased. Compared with carcinoma group, the expression levels of miR-19a （t = 3. 980, P 〈 0. 001 ） and TNF-α mRNA （t = 6. 052, P 〈 0. 001 ） in the study group were significantly lower. The expression of miR-19a and TNF-α was correlated with age, sex, course of disease and clinical phenotype （P 〈 0.05 ）. TNF-α mRNA level increased with the expression level of miR-19a in oral mucosal tissue from OLP patients, there was an positive corre- lation （r = 0. 495 ,P 〈 0.05 ）. Conclusion The expression of miR-19a and TNF-α mRNA in oral mucosal tissue from OLP patients was significantly increased, which was related to age, sex, duration and clinical phenotype.