基于肠外翻-离体血管联用方法的银丹心脑通软胶囊血管活性评价

Vasoactivity evaluation of Yindan Xinnaotong Capsule based on everted gut sacs coupled with isolated vascular

目的:建立肠外翻-离体血管联用方法考察银丹心脑通软胶囊肠吸收液对血管的舒张作用。方法:应用外翻肠囊法制备银丹心脑通软胶囊肠吸收液,采用大鼠离体主动脉环模型,观察肠吸收液对离体血管的舒张作用,并采用UPLC-QQQ对肠吸收液中人参皂苷Rb1、人参皂苷Re、人参皂苷Rd、人参皂苷Rg1、三七皂苷R1、银杏内酯A、银杏内酯B、银杏内酯C、白果内酯、芦丁和丹参酮ⅡA进行定量分析。结果:银丹心脑通软胶囊肠吸收液在100-1 600μL的浓度范围内均具有浓度依赖性舒张作用。且11个成分均能很好被肠囊吸收,所有成分累计肠吸收量在高浓度下含量最高,低浓度下最低。结论:本研究所建立的方法简单、可控,更能反应样品的真实生物活性,可为银丹心脑通软胶囊质量评价提供参考。

Objective： To establish a method coupled with everted gut sacs and isolated vascular, and study the effects of Yindan Xinnaotong Capsule on intestinal absorption of vasoactivity. Methods： The everted intestinal sac method was performed to prepare the intestinal absorption solution. The isolated rat aortic ring model was used to observe the effects of intestinal absorption on the vasoactivity in vitro. Additionally, the main compounds（ginsenoside Rg1, ginsenoside Rb1, ginsenoside Re, ginsenoside Rd, notoginsenoside R1, ginkgolide A, ginkgolide B, ginkgolide C, bilobalide, rutin, and tanshinone IIA） in intestinal absorption solution were determined by UPLC-QQQ. Results： The intestinal absorption liquid of Yindan Xinnaotong Capsule in the concentration range of 100-1 600 L had a concentration-dependent vasodilation. Eleven constituents could be absorbed by gut sacs. The cumulative amount of intestinal absorption of all components was the highest in high concentration and the lowest in low concentration. Conclusion： The method is simple and controllable. It can reflect the true biological activity of samples, which provide a reference for the quality evaluation of Yindan Xinnaotong Capsule.