补阳还五汤对矽尘所致肺纤维化大鼠血清PCⅢ和Ⅳ-C含量的影响

Effect of Buyang Huanwu Decoction on contents of PC Ⅲ and Ⅳ-C in blood serum of pulmonary fibrosis rats caused by silica dust

目的探讨补阳还五汤对矽尘所致肺纤维化大鼠血清PCⅢ、Ⅳ-C表达的影响。方法将144只健康雄性SD大鼠采用随机数字表法分为对照组、模型组、给药组(包括泼尼松组和补阳还五汤高、中、低剂量组),每组24只。对照组气管内滴注生理盐水,模型组与给药组气管内滴注矽尘悬液制作矽肺大鼠模型,造模第2天起,给药组给予相应药物灌胃,于造模后第7、14、28天分3批处死动物,每次8只。检测各组大鼠血清PCⅢ、Ⅳ-C的含量。结果与对照组比较,各组大鼠血清PCⅢ、Ⅳ-C的含量逐渐升高,模型组最为明显(P<0.01);与模型组比较,各给药组大鼠血清PCⅢ、Ⅳ-C含量均降低(P<0.01);与泼尼松组比较,补阳还五汤中、高剂量组第14、28天时PCⅢ含量明显降低,补阳还五汤中剂量组第14、28天及高剂量组第28天时,Ⅳ-C含量明显降低(P<0.05)。结论补阳还五汤可以有效降低染尘大鼠血清PCⅢ、Ⅳ-C的含量抑制矽肺肺纤维化。

Objective To explore the effect of Buyang Huanwu decoction on contents of PC Ⅲ and Ⅳ in blood serum of pulmonary fibrosis rats caused by silica dust. Methods Totally 144 healthy male SD rats were randomly divided into six groups ： control group, model group, prednisone group, Buyang H uanwu decoction high-, medium-, low-dose groups, 24 rats in each group. In addition to the control group the other groups were made of rat model of silicosis by disposable tracheal drip silica dust suspension,and control group of tracheal infusion of the same amount of normal saline. From the second day, all the rats received a gavage by related medicine. Eight rats in each group were killed respectively on day 7,14 and 28 after intratracheal instillation. The contents of PCⅢ and Ⅳ-C in blood serum of experimental rats were tested by ELISA method. Results Compared with the control group,the contents of PC]II and IV-C in blood serum of other groups increased gradually,and that of the model group was the most obvious（ P 〈0.01）. Compared with the model group, each medicine group had different decreases in the contents of PCⅢ and Ⅳ-C in blood serum（ P 〈0.01）. Compared with the prednisone group,the contents of PCⅢ in blood serum of Buyang Huanwu decoction medium-dose group obviously decreased on the 14th and 28th day（ P 〈0.05） ,as well as that of Buyang Huanwu decoction high-dose group on the 28th day （ P 〈0.05）. The contents of IV-C in blood serum of Buyang Huanwu decoction medium-dose group obviously decreased on the 14th and 28th day （ P 〈0. 05） ,as well as that of Buyang Huanwu decoction high-dose group on the 28th day （ P 〈0.05）. Conclusion Buyang Huanwu decoction can effectively reduce contents of PCⅢ and Ⅳ-C in blood ser um of pulmonary fibrosis rats caused by silica dust.